Protein Info for AO353_20845 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): uridine/adenosine nucleosidase (EC 3.2.2.3; EC 3.2.2.7)
Rationale: Important on adenosine and uridine; annotated by SEED as for uridine and inosine; fitness defect on uridine is subtle but is confirmed by observations for close homologs (SEED_correct)
Original annotation: nucleoside hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 34 to 332 (299 residues), 312.9 bits, E=1.4e-97

Best Hits

KEGG orthology group: K01239, purine nucleosidase [EC: 3.2.2.1] (inferred from 93% identity to pfo:Pfl01_1925)

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of pyrimidine ribonucleosides salvage (7/10 steps found)
• pyrimidine ribonucleosides salvage II (1/2 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.1 or 3.2.2.3 or 3.2.2.7

Sequence Analysis Tools

See A0A0N9WN92 at UniProt or InterPro

Protein Sequence (342 amino acids)

>AO353_20845 uridine/adenosine nucleosidase (EC 3.2.2.3; EC 3.2.2.7) (Pseudomonas fluorescens FW300-N2E3)
MYRYAQRLHHLIRSLLLLSLLTATGAHAAEKIDLIIDTDPGADDVVALLFALASPEELNI
RALTTVAGNVRLDKTSRNARLAREWAGREDVPVYAGAPKPLMRTPIYAENIHGKEGLSGV
TVHEPKKGLAKGDAVSYLIDTLRAAKPHSITIAMLGPQTNLALALIQDPEITQGIKEVVI
MGGAHFNGGNITPVAEFNLFADPHAAEVVLKSGVKLTYLPLDVTHKILTSDARLKQIAAL
NNNASKLVGDILNEYVKGDMEHYGIPGGPVHDATVIAYLLKPELFTGRAVNVVVDSREGP
TFGQTVVDWYDGLKAPKNAFWVANGDAQGFFDLLTARLARLK