Protein Info for AO353_20365 in Pseudomonas fluorescens FW300-N2E3

Annotation: hydroxymethylglutaryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 7 to 275 (269 residues), 172.5 bits, E=6.7e-55

Best Hits

Swiss-Prot: 78% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 92% identity to pba:PSEBR_a1847)

MetaCyc: 78% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• mevalonate degradation (1/2 steps found)
• ketogenesis (3/5 steps found)
• cis-geranyl-CoA degradation (4/9 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Geraniol degradation
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4

Sequence Analysis Tools

See A0A0N9WLY8 at UniProt or InterPro

Protein Sequence (299 amino acids)

>AO353_20365 hydroxymethylglutaryl-CoA lyase (Pseudomonas fluorescens FW300-N2E3)
MPLPTHVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEIGSFVSPKWVPQMA
GSAEVFAQIQRKPGVTYGALAPNLRGFEDAVAAGVKEVAVFAAASESFSQRNINCSISES
LARFVPIMEAARQHGVSVRGYVSCVLGCPYEGDVAPEQVALVARELYAMGCYEVSLGDTI
GTGTAGATRKMFEVVSADVPRDKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGC
PYAKGASGNVATEDVLYLLNGLGIETGVDMDKLILAGQQICNVLGRPTGSRVAKARSAQ