Protein Info for AO353_20360 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF14226: DIOX_N" amino acids 24 to 123 (100 residues), 48 bits, see alignment E=1.9e-16 PF03171: 2OG-FeII_Oxy" amino acids 169 to 286 (118 residues), 54.1 bits, see alignment E=1.7e-18

Best Hits

Swiss-Prot: 80% identical to EFE_PSESH: 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (efe) from Pseudomonas savastanoi pv. phaseolicola

KEGG orthology group: None (inferred from 63% identity to fri:FraEuI1c_2575)

MetaCyc: 80% identical to 2-oxoglutarate-dependent ethylene/succinate-forming enzyme (Pseudomonas savastanoi pv. phaseolicola)
RXN-12536 [EC: 1.14.20.7]; RXN-14542 [EC: 1.14.20.7, 1.14.11.41]; 1.13.12.19,1.14.11.- [EC: 1.14.20.7, 1.14.11.41, 1.13.12.19]

Predicted SEED Role

"small molecule metabolism"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.12.19 or 1.14.11.41 or 1.14.20.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VX62 at UniProt or InterPro

Protein Sequence (353 amino acids)

>AO353_20360 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
MTDLQTFKLPDTVTGLTADISLGVKLIHAWQTDGIFQIETEEEQDYKTQAAITASKDFFK
KKMEFKSSRISDLTYSGYIASREEVTAGKADYSEIFTICKDLPETDPHVEAGWPCHGPVP
WPDDTYRISMKAFMDELGLAGEKLLKLTALGLKLPITTFTDLTRDGWHHMRVLRFPTSTS
ESSRGIGAHTDYGLLVIAAQDDVGGLYIRPPVEGEKRNRNWLPGESSAGLFEHEEPWTFV
KPTPKVWTVFPGDILQFMTNGRLLSTPHKVKLNTRERFACAYFHEPNFEAVARPLFDPDS
KERIHYGEHFTHMFMRCYPKRITTQRIHAEKRLRHLEDLKKSETRTARYATIS