Protein Info for AO353_20220 in Pseudomonas fluorescens FW300-N2E3

Annotation: 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 679 PF00724: Oxidored_FMN" amino acids 9 to 334 (326 residues), 295.3 bits, see alignment E=3.1e-91 PF03486: HI0933_like" amino acids 379 to 418 (40 residues), 29.6 bits, see alignment 1.4e-10 PF07992: Pyr_redox_2" amino acids 379 to 645 (267 residues), 88.6 bits, see alignment E=2.4e-28 PF00070: Pyr_redox" amino acids 379 to 417 (39 residues), 23 bits, see alignment 4.3e-08 PF00890: FAD_binding_2" amino acids 380 to 417 (38 residues), 24.3 bits, see alignment 8.1e-09 PF12831: FAD_oxidored" amino acids 380 to 416 (37 residues), 27.7 bits, see alignment 8.2e-10 PF13450: NAD_binding_8" amino acids 382 to 417 (36 residues), 35.8 bits, see alignment 3.6e-12

Best Hits

Swiss-Prot: 63% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 96% identity to pba:PSEBR_a1822)

MetaCyc: 63% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W8F4 at UniProt or InterPro

Protein Sequence (679 amino acids)

>AO353_20220 2,4-dienoyl-CoA reductase (Pseudomonas fluorescens FW300-N2E3)
MTAAHYPHLLAPLDLGFTTLRNRTLMGSMHTGLEEKPGGFERMAAYFAERARGGVGLMVT
GGIGPNDEGGVYSGAAKLTTLDEALKHQVVTRAVHEAGGKICMQILHAGRYAYSPKQVAP
SAIQAPINPFKPKELDEEGIEKQISDFVTCSTLAQKAEYDGVEIMGSEGYFINQFLAAHT
NHRTDRWGGSYENRMRLPVEIVRRVREAVGPNFIIIFRLSMLDLVEGGSTWEEIVQLAKA
IEQAGATIINTGIGWHEARIPTIATKVPRAAFSKVTAKLRGEVGIPLITTNRINTPEVAE
QILAEGDADMVSMARPFLADPEFVNKAAAGRSDEINTCIGCNQACLDHTFGGKLTSCLVN
PRACHETELNYLPVQQIKKIAVVGAGPAGLSAATVAAERGHQVTLFDSASEIGGQFNVAK
RVPGKEEFFETLRYFKRKLQTTNVEVCLNTRVDVAQLVEGGYDEVILATGIAPRVPAIPG
VEHAKVLSYLDVILERKPVGKSVAVIGAGGIGFDVSEFLVHQGVATSQDREAFWKEWGID
THLEARGGVAGIKAEPHAPARQVFLLQRKKSKVGDGLGKTTGWIHRTGLKNKHVQMLNSV
EYLKIDDEGLHIRIGETGEPQVLPVDNIVICAGQDPLRELHDGLVAAGQNVHLIGGADVA
AELDAKRAINQGSRLAAEL