Protein Info for AO353_20080 in Pseudomonas fluorescens FW300-N2E3
Annotation: nitric oxide synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 72% identity to pba:PSEBR_a2540)Predicted SEED Role
"Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
- assimilatory sulfate reduction I (4/4 steps found)
- assimilatory sulfate reduction III (3/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.2
Use Curated BLAST to search for 1.8.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9W8E0 at UniProt or InterPro
Protein Sequence (740 amino acids)
>AO353_20080 nitric oxide synthase (Pseudomonas fluorescens FW300-N2E3) MLRQFHSLPGLVAALLVMLLAISGAILSINPALERLSATVPASGQVNVAELAGRVANHYP GVEKIRRTASGSVIVYFTQDGQTGAERVDPLTGAGVASYTPSAFTRWVKDLHRSLLSGTP GHAAAGIGALFILILSISGAALLAQRLGGWRRVMRPLRGSFNQRWHAEVGRLAILGLLLS ALTGLYMSATTFGLIPDGTQREPVFPSTLSSGTAAPVATLPALLATDLNDLRELAYPSVG DPEDFFSLRTAQGDGYVDPTNGALLSYVPHDSMRNAYEFIYQLHTGENLWWLGLFLGVCA LSVPLMSVTGALLWWRRRQSQPKIRHNSGAQNADTVILVGSESNSTWGFANTLHEALQQT GHRVHTAPMNRLAVEYRSAQRLFILTATTGDGDAPASASQFLKRLANARLKPGLGFAVLG FGDRQFPKFCQFAKDAQAALLASGGTQLLELETINRQSAQAFTRWGSAVGRLLGHELTLV HVPRLPETTTLALIDRTHYGEHVNAPTQILRFNAAATSHSPGELKHRRNSGLPRFQAGDL VGVLPPGSRVPRYYSLASGSDDGVLEICVRKHADGVCSGFLHDLPLGGQIEAFIQPNPEF RPGRGKQPVILIGAGTGIGPLAGFIRNNTARKPMHLYWGGRHPASDFLYEPELNRYLADQ RLTRLRAAFSQTHERSYVQDRLIADALALRRLIEKGAHVLVCGSREMAKGVMNALDEVLA PLDLNVLTLKAQGRYREDVY