Protein Info for AO353_19800 in Pseudomonas fluorescens FW300-N2E3

Annotation: protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 PF01136: Peptidase_U32" amino acids 79 to 338 (260 residues), 293.1 bits, see alignment E=1.3e-91 PF16325: Peptidase_U32_C" amino acids 355 to 436 (82 residues), 69.6 bits, see alignment E=2.1e-23

Best Hits

Swiss-Prot: 73% identical to YEGQ_ECOLI: Uncharacterized protease YegQ (yegQ) from Escherichia coli (strain K12)

KEGG orthology group: K08303, putative protease [EC: 3.4.-.-] (inferred from 93% identity to pfo:Pfl01_3718)

Predicted SEED Role

"Putative protease"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WYC4 at UniProt or InterPro

Protein Sequence (441 amino acids)

>AO353_19800 protease (Pseudomonas fluorescens FW300-N2E3)
MPLATPELLAPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFDHANLALGIREAQAQ
GKRFYVVVNIAPHNAKLKTFLKDLEPVIAMAPDALIMSDPGLIMLVRRHFPQMPIHLSVQ
ANTVNWASVEFWQQQGLSRIILSRELSLEEIAEIRQQVPAMELEVFVHGALCMAYSGRCL
LSGYMNKRDANQGTCTNACRWKYSAQPATENVVGEIVQAFIPEPTLGIGTPTDQVFLLQE
ANRPDELMPAFEDEHGTYIMNAKDLRAVQHVARLTQMGVHSLKIEGRTKSHFYCARTTQV
YRRAIDDAVAGREFDRSLMTDLESLAQRGYTEGFLRRHVHDEYQNYQNGSSVSERQQFVG
ELTGERRDRLAEVKVKNRFGLGDHMELMTPKGNFHFDLHQLQSAKGEAIEVAPGDGHTVY
LPIPDAVDLKFGLLMRDVRGD