Protein Info for AO353_19715 in Pseudomonas fluorescens FW300-N2E3

Annotation: disulfide bond formation protein DsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details amino acids 62 to 83 (22 residues), see Phobius details amino acids 90 to 110 (21 residues), see Phobius details amino acids 117 to 141 (25 residues), see Phobius details amino acids 148 to 171 (24 residues), see Phobius details amino acids 178 to 199 (22 residues), see Phobius details amino acids 211 to 231 (21 residues), see Phobius details amino acids 243 to 260 (18 residues), see Phobius details amino acids 281 to 305 (25 residues), see Phobius details amino acids 315 to 337 (23 residues), see Phobius details amino acids 345 to 363 (19 residues), see Phobius details amino acids 383 to 402 (20 residues), see Phobius details amino acids 414 to 431 (18 residues), see Phobius details amino acids 495 to 516 (22 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 24 to 511 (488 residues), 391.3 bits, see alignment E=3.2e-121 PF07690: MFS_1" amino acids 29 to 423 (395 residues), 140.1 bits, see alignment E=4.5e-45

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_3794)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VWV3 at UniProt or InterPro

Protein Sequence (529 amino acids)

>AO353_19715 disulfide bond formation protein DsbA (Pseudomonas fluorescens FW300-N2E3)
MSRALTAQAVPFNAANMATATKVFAFATMCIGMFIALLDIQIVSASLRDIGGGLSAGTDE
TAWVQTSYLIAEIIVIPLSGWLSRVFSTRWLFCVSAVGFTLASLLCGAAWNIQSMIAFRA
LQGFLGGSMIPLVFTTAFFFFTGKQRVIAAATIGAVASLAPTLGPVIGGWITDVSSWHWL
FYINLVPGIFVAVAVPMLVKIDQPELSLLKGADYLSMVFMALFLGCLEYTLEEGPRWNWF
SDQTILTTAWISGLAGLAFIGRSLHVNNPIVDLRALKDRNFALGCFFSFVTGIGLFATIY
LTPLFLGRVRGYGALDIGLAVFSTGVFQIMAIPLYAFLANRVDLRWIMMTGLGLFALSMW
DFSPITHDWGARELILPQALRGIAQQLAVPPAVTLTLGGLAPARLKHASGLFNLMRNLGG
AIGIAACATILNDRTNLHFTRLAEHLNSSNEAMNQWLSQVGSNFAALGQSGDAGVTASLH
QLWLLTYREAQTQTYGDAFLMIMLCFIVATAMVPLMRKVAPPAAPVADH