Protein Info for AO353_19680 in Pseudomonas fluorescens FW300-N2E3

Annotation: mechanosensitive ion channel protein MscS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 34 to 52 (19 residues), see Phobius details amino acids 75 to 95 (21 residues), see Phobius details amino acids 107 to 126 (20 residues), see Phobius details amino acids 132 to 155 (24 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 151 to 217 (67 residues), 66.6 bits, see alignment E=1.7e-22 PF00027: cNMP_binding" amino acids 351 to 433 (83 residues), 61.1 bits, see alignment E=8.2e-21

Best Hits

KEGG orthology group: None (inferred from 83% identity to pfo:Pfl01_1867)

Predicted SEED Role

"Mechanosensitive ion channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VXE9 at UniProt or InterPro

Protein Sequence (478 amino acids)

>AO353_19680 mechanosensitive ion channel protein MscS (Pseudomonas fluorescens FW300-N2E3)
MLSLLTDHPLFCALALILLDILLWRLVSVSHWQLVVRVVIFALFSAVLFNEGMNPMDVAP
WPENVPLHLAATGLQIGWWLFGARTLTVLLGTVMMQRVGHTGRLLQDLLGAVIFLIAIIA
ALAYVLDLPVKGVLATSGALAIIVGLALQSTLSDVFSGIVLNTTKPYQLDDWISIDGTEG
RVTDIDWRATRLQTSQGSMAVIPNSLAAKAKIINFSRPSDIFGITISLQLSPHARPHTVI
EALERAMQGCRPLLSKPAPSVSLKSSSASGVEYEIIGFVSSMGQKREVRNLLFDLAFRHL
RASGVSLLSTAETTTLPGLSRPRALLESSSIFSTLRSEEKDTFSQNMTLITFRAGEMILP
AGEVSDHLFIIESGVVSVALSRGGKKFEAGRMGPGEVIGEAGILSDESVPADFSAKTFCT
LYRIEKEYLKPCLDARHDINEAMQALLDFRLHTAQTLTQEVPKVVEKKGFLQWLRSRA