Protein Info for AO353_19590 in Pseudomonas fluorescens FW300-N2E3

Annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 48 to 66 (19 residues), see Phobius details amino acids 72 to 92 (21 residues), see Phobius details amino acids 104 to 125 (22 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 344 to 364 (21 residues), see Phobius details PF01370: Epimerase" amino acids 186 to 405 (220 residues), 82.2 bits, see alignment E=8.3e-27 TIGR01777: TIGR01777 family protein" amino acids 186 to 476 (291 residues), 316.2 bits, see alignment E=1.3e-98 PF05368: NmrA" amino acids 186 to 220 (35 residues), 22 bits, see alignment 2e-08 PF08338: DUF1731" amino acids 433 to 479 (47 residues), 66.5 bits, see alignment 2.7e-22

Best Hits

KEGG orthology group: K07071, (no description) (inferred from 92% identity to pba:PSEBR_a216)

Predicted SEED Role

"Cell division inhibitor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W885 at UniProt or InterPro

Protein Sequence (480 amino acids)

>AO353_19590 NAD-dependent dehydratase (Pseudomonas fluorescens FW300-N2E3)
MADFSLLDWALTLLIAQAVLGALDTLYHHELTVALPYRHSARLELSIHALRSCFYGILFL
GMAHLAFQGTWAIVIALLFALEIGLTLWDFVVEDRSRKLPAIERIMHTVLAVNGGAFFAL
YGVQLAQWATLPTALSPIDFGWRGWVLTLFAIGVTASGIRDGLAALRMQREGKPANPFAG
LAYKRVLVTGGTGFIGETLVNQLLDAGHTVSVLARDPLKAAYLFDGRARCLRSLSKLGYN
ETFDVVINLAGAPVAGPRWSAKRQAQLLASRVNTTQGLMTWLKNARHKPALWVQASAIGF
YGVRDASESLDESATKGEGFMAELCARWEAAAQPASELGVRQVVMRLGVVFGPGGALLPL
LIPFRLGFGGRMGDGQQIMSWVHRDDVIQVIARAFDDNSLSGTYNLVAPDAVSQAVFADS
VGKVLKRPVWFHIPAAPVRTLAGEMAQLFFDGQRVVPVRLTEAGYTFRYPTLDAALRDLA