Protein Info for AO353_19515 in Pseudomonas fluorescens FW300-N2E3

Annotation: haloacid dehalogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 PF00702: Hydrolase" amino acids 3 to 178 (176 residues), 96.2 bits, see alignment E=5e-31 PF13419: HAD_2" amino acids 5 to 183 (179 residues), 109.3 bits, see alignment E=3.8e-35 PF12710: HAD" amino acids 6 to 132 (127 residues), 43.6 bits, see alignment E=7e-15 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 72 to 183 (112 residues), 47.1 bits, see alignment E=2.8e-16

Best Hits

KEGG orthology group: None (inferred from 80% identity to pfl:PFL_4122)

Predicted SEED Role

"Beta-phosphoglucomutase (EC 5.4.2.6)" in subsystem Maltose and Maltodextrin Utilization or N-Acetyl-Galactosamine and Galactosamine Utilization or Trehalose Uptake and Utilization (EC 5.4.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1C8 at UniProt or InterPro

Protein Sequence (223 amino acids)

>AO353_19515 haloacid dehalogenase (Pseudomonas fluorescens FW300-N2E3)
MLNALLFDLDGTLTDTDALHLLALQQLLLEEDGRVFSKAEFEAHVSGQANANMCRYLFPD
RSVAEREAFAERKEVRFRELSPQLTPMAGLVRLLDFAKAQDIGIAVVTNAPRANADHMLR
ALGLRERFEHIVVAEELPRAKPDPLPYLTGLERLQASAKVTLAFEDSIPGLTAAVNAGIF
TVGLVTSQRRETLLAAGAHLVIEDFDDPQLWAVIDRMLSRQGL