Protein Info for AO353_19345 in Pseudomonas fluorescens FW300-N2E3

Annotation: RNA polymerase subunit sigma-70

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 182 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 19 to 168 (150 residues), 78.3 bits, see alignment E=2.7e-26 PF04542: Sigma70_r2" amino acids 24 to 83 (60 residues), 48.1 bits, see alignment E=1.3e-16 PF08281: Sigma70_r4_2" amino acids 116 to 168 (53 residues), 50.1 bits, see alignment E=2.6e-17 PF04545: Sigma70_r4" amino acids 121 to 170 (50 residues), 27.5 bits, see alignment E=2.7e-10

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 93% identity to pfl:PFL_4190)

Predicted SEED Role

"Sigma factor PvdS, controling pyoverdin biosynthesis" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WKL7 at UniProt or InterPro

Protein Sequence (182 amino acids)

>AO353_19345 RNA polymerase subunit sigma-70 (Pseudomonas fluorescens FW300-N2E3)
MTEPVTTSRCDSPLLQAFVNNRLILVKIAARITGCRSRAEDVVQDAFFRLQSAPQITSSF
KAQLSYLFQIVRNLAIDHYRRQALEQKYSGTEEEGLNVVIHDASPETSHINFSILENIAD
ALGQLPSRTRYAFEMYRLHGVPQKDIAKELGVSPTLVNFMIRDALVHCRKVSGKRSDTFA
RR