Protein Info for AO353_19225 in Pseudomonas fluorescens FW300-N2E3

Annotation: sodium:proton antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 27 to 45 (19 residues), see Phobius details amino acids 55 to 72 (18 residues), see Phobius details amino acids 82 to 104 (23 residues), see Phobius details amino acids 110 to 127 (18 residues), see Phobius details amino acids 146 to 166 (21 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 213 to 230 (18 residues), see Phobius details amino acids 235 to 254 (20 residues), see Phobius details amino acids 267 to 285 (19 residues), see Phobius details amino acids 291 to 312 (22 residues), see Phobius details amino acids 323 to 342 (20 residues), see Phobius details amino acids 353 to 374 (22 residues), see Phobius details TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 10 to 281 (272 residues), 252.1 bits, see alignment E=3.5e-79 PF00999: Na_H_Exchanger" amino acids 10 to 370 (361 residues), 220.2 bits, see alignment E=6.1e-69 PF02254: TrkA_N" amino acids 405 to 518 (114 residues), 80.4 bits, see alignment E=2e-26 PF02080: TrkA_C" amino acids 581 to 645 (65 residues), 28.8 bits, see alignment E=1.4e-10

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 80% identity to pfl:PFL_4229)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WLF6 at UniProt or InterPro

Protein Sequence (651 amino acids)

>AO353_19225 sodium:proton antiporter (Pseudomonas fluorescens FW300-N2E3)
VFVNLLIILASSLVVIALFRRLQLPPVLGYLCVGLLVGPGAFGWVNDNQDLPDLAELGVV
FLLFSLGLEFSLSKMLALRRVVFGLGSLQVLCSGVLLGGLLMLLGMPAPPAMLLGAGLGL
SSTAIVSKELSSLGEIFSRHGQNAIGVLLFQDVVAVLLLTLVPVFAGSSDQSWYWALPIT
LGKTVLSFVGLLLTSRWLLPRLFHEVATSRSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHGLLILGLTLGLL
LIKGSIVAALVKWRGSNGETAWRSGLALAQGGEFCFALMAQMQQNKLIPAELSGLLLAAT
FCSMLLTPLLLRAAPGIAARLHRKPDKEAQLDQVAALNAELHGHVVICGYGRVGQSIGRF
LRREGQPFVALDDDPVRVHQAAAGESCVHYGDSRRVELLGAVGLERARLLVIAVDKTDIA
MTVLKAARQLNAQVPILVRTRDDSQLAELKAAGANEVVPELLESSLMLASHALLMLGLPH
PQVQERVDEVRHGRYRLLHGFYHGAQSNLLDSRGQPKVLMYAVNLTMDAHACGRPLNELA
LEALGVDVQAIQRNGADLAVEGVTCLLAGDRVLMSGTLSAIEACEARLLAG