Protein Info for AO353_19185 in Pseudomonas fluorescens FW300-N2E3

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1209 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 540 to 560 (21 residues), see Phobius details PF00497: SBP_bac_3" amino acids 67 to 277 (211 residues), 107.4 bits, see alignment E=2.8e-34 amino acids 306 to 525 (220 residues), 116.7 bits, see alignment E=4.1e-37 TIGR00229: PAS domain S-box protein" amino acids 581 to 699 (119 residues), 24.7 bits, see alignment E=1.1e-09 PF00989: PAS" amino acids 581 to 693 (113 residues), 25.5 bits, see alignment E=3.9e-09 PF08448: PAS_4" amino acids 588 to 698 (111 residues), 42.8 bits, see alignment E=2e-14 PF00512: HisKA" amino acids 716 to 782 (67 residues), 65.3 bits, see alignment 1.4e-21 PF02518: HATPase_c" amino acids 831 to 946 (116 residues), 92.2 bits, see alignment E=1.1e-29 PF00072: Response_reg" amino acids 970 to 1084 (115 residues), 81.2 bits, see alignment E=2.2e-26 PF01627: Hpt" amino acids 1115 to 1197 (83 residues), 25.5 bits, see alignment 4.4e-09

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 70% identity to pfs:PFLU1739)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VX99 at UniProt or InterPro

Protein Sequence (1209 amino acids)

>AO353_19185 histidine kinase (Pseudomonas fluorescens FW300-N2E3)
MPRCLKDYLLYLSAGLCLSTSVSGAGSGVGNYALLSRSTAGYMDVQLDKSQRLWLQNKRE
LILGTSAPDYPPFDLTISGHDYEGFTADYAGVLAQSLGLPIKVRRYLSREAAIEALENGD
VDLLGTANSFEERRSSIVLSAPYAIDQPVLVTRNAENRSLTEGLADMRLSMVYHYLPLEE
VKAQYPKAIITSYPSYQNAINAVAFDQADVFLGDTISTHFMINKGYLKNVRMANFGKHEA
YGFSFAVNKDNPDLLAIINAVLKEVPASERENIAKRWSAGSDILLTDHKLQLTNREERWL
AQHPVVRVVVNDAFAPLTFFDADESFRGITADLLELIRLRTGLRFEIHRSQSDSEMISQV
KGNQADLIAAMIPSAEREAQLSFTRPYLENSFVLLTRKSTSSPINLEQLNGKSLAITQGN
PLLDYLHRAYPTIQLVETPDSFSAVELLAKGQVEGAVNSLVIANYFISSQVFEDALQISA
TVGTQQAAFSLATARDATELHSILDKALLSIAPDELGIINSRWRGYTGTANSAWRNYHRL
FYQLVIGSGLLLLISLAWNAHMRRQIKQRQKAERALNDQFEFMRTLVNGTPHPIYVRDRE
GLLQSCNDSYLEAFCAKREDVIGKSLMQGAMSNEFEAREYLADYQRVVAEGVPLILDRPL
HIGRKRLTIYHWILPYRDSTGEVQGIIGGWIDISERRQLFDELRSAKEQADKANRAKSTF
LATMSHEIRTPMNAVIGMLELTLKRTDQSHLDRPAIEVAYSSAKDMLELIGDILDIARIE
SGHLSLAPERVNLGDTIESVVRVFDGLARQKNLCLVLEFPTSAEQPDVLIDPLRFKQILS
NLISNAIKFTEHGQVRIKVNLQPASESDRLQMELWVNDTGIGISEDDQRRLFEPFTQADN
TGHLARSGAGLGLVISRNLCEMMGGCVQLTSSPGVGTQVRISLPMAALPDAVVLDTPQPV
IANASATLNVLVVDDHPANRLLMCQQLGFLGHRSVTANNGEAGFQAWKEGHFDLVIADCN
MPLMNGYELTRAIRQTERDEQRPPCTVLGFTANAQPEEKHRCRQAGMNDCLFKPISLTTL
GQRLEALKPIIEDQAFHLDGLQLLTGGDPQLVQRLLAELFNSNRLDRQELLALPPASGPQ
AFIDMAHKIKGAARIVQASRLIDGCEALERVCHENFCLDLLEARCKGIEHAMLELEQALQ
RQIHHHANV