Protein Info for AO353_18805 in Pseudomonas fluorescens FW300-N2E3

Annotation: chemotaxis protein CheY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 PF00072: Response_reg" amino acids 5 to 109 (105 residues), 75.8 bits, see alignment E=3.1e-25 PF01339: CheB_methylest" amino acids 194 to 370 (177 residues), 208.1 bits, see alignment E=8.5e-66

Best Hits

Swiss-Prot: 93% identical to CHEB2_PSEPF: Protein-glutamate methylesterase/protein-glutamine glutaminase 2 (cheB2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03412, two-component system, chemotaxis family, response regulator CheB [EC: 3.1.1.61] (inferred from 93% identity to pfo:Pfl01_1567)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VWH1 at UniProt or InterPro

Protein Sequence (375 amino acids)

>AO353_18805 chemotaxis protein CheY (Pseudomonas fluorescens FW300-N2E3)
MAVKVLVVDDSGFFRRRVSEILSADPSIQVVGTATNGKEAIDQALALKPDVITMDYEMPM
MDGITAVRHIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDFLPKNFEDISRNPEKVKQ
LLCEKVHSISRSNRRFSAYSAPAPAAAPAPAPAPSSFGSSAPAPVRPAPTPIPARAPVAS
ASSPAPKRKAYKLVAIGTSTGGPVALQRVLTQLPANFPAPIVLIQHMPAAFTKAFAERLD
KLCRISVKEAEDGDILRPGLALLAPGGKQMMIDGRGAVKILPGDERLNYKPCVDITFGSA
AKSYGDKVLAVVLTGMGADGREGARLLKQGGSAVWAQDEASCVIYGMPMAIVKADLADAI
YGLDDIGKHLAEACI