Protein Info for AO353_18800 in Pseudomonas fluorescens FW300-N2E3

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 transmembrane" amino acids 606 to 624 (19 residues), see Phobius details PF01627: Hpt" amino acids 8 to 100 (93 residues), 64.9 bits, see alignment E=1.3e-21 PF02895: H-kinase_dim" amino acids 361 to 420 (60 residues), 58.7 bits, see alignment 1.2e-19 PF02518: HATPase_c" amino acids 467 to 607 (141 residues), 66.8 bits, see alignment E=4.5e-22 PF01584: CheW" amino acids 614 to 741 (128 residues), 96.7 bits, see alignment E=1.9e-31

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 89% identity to pfo:Pfl01_1566)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0F9 at UniProt or InterPro

Protein Sequence (747 amino acids)

>AO353_18800 chemotaxis protein CheA (Pseudomonas fluorescens FW300-N2E3)
MSFGADEEILQDFLVEAGEILEQLSEQLVELESRPDDADLLNAIFRGFHTVKGGAGFLQL
NELVECCHIAENVFDILRKGERRVDSELMDVVLEALDAVNSMFSEVRERAPITAATPELL
AALARLAEPQTADVAQPVAEVVEAPAAESESGDITDNEFELLLDSLNAVKAQAESGAPVA
PAPEAAAGSDEITDAEFESLLDQLHGKGQFAPDAVAAAPAAPAAPKAAGDSSDITDDEFE
ALLDQLHGKGNFAVDALESAIAAVPAPAAPVAAAVGSDLISDHEFESLLDELHGKGKFTE
VGTGSAASVAAPVAKAAAPAPVAKAPEPKAETPKPAAAAAPAPARAAASPPPEKPASEAE
TTVRVDTARLDEIMNMVGELVLVRNRLVRLGLNSGDEAMSKAVSNLDVVTADLQTAVMKT
RMQPIKKVFGRFPRLVRDLARQLKKEINLELVGEETDLDKNLVEALADPLVHLVRNSVDH
GIEEPADREAMGKPRSGKVILSAEQEGDHILLSISDDGKGMDADVLRGIAVKKGLMDKDA
ADRLSESDCFNLIFAPGFSTKTEISDVSGRGVGMDVVKTKIAQLNGTINIYSTKGKGSKI
VIKVPLTLAIMPTLMVMLGNQAFAFPLVNVNEIFHLDLSRTNVVDGQEVVIVRDKALPLF
YLKRWLVSSAAHEEQREGHVVILSVGTQRIGFVVDQLVGQEEVVIKPLGKMLQGTPGMSG
ATITGDGRIALILDVPSMLKRYAARRI