Protein Info for AO353_18635 in Pseudomonas fluorescens FW300-N2E3

Annotation: branched-chain alpha-keto acid dehydrogenase subunit E2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 PF00669: Flagellin_N" amino acids 5 to 141 (137 residues), 149.5 bits, see alignment E=6.4e-48 PF00700: Flagellin_C" amino acids 395 to 480 (86 residues), 90.5 bits, see alignment E=7e-30

Best Hits

Swiss-Prot: 54% identical to FLICB_PSEAE: B-type flagellin (fliC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02406, flagellin (inferred from 68% identity to ppg:PputGB1_3939)

Predicted SEED Role

"Flagellin protein FlaB" in subsystem Flagellum or Flagellum in Campylobacter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0F5 at UniProt or InterPro

Protein Sequence (481 amino acids)

>AO353_18635 branched-chain alpha-keto acid dehydrogenase subunit E2 (Pseudomonas fluorescens FW300-N2E3)
MALTVNTNLASLTVQNNLNKASSSLQTSMQRLSSGLRINSAKDDAAGLQISNRLTSQING
LGTAIKNAGDGISIAQTAEGAMQESTNILQKMRTLALASANGSPSAEDRKSNNAEYSALT
SELTRIATTTTFGGRKILDGSFGTTNFQVGANANETIGMTLGDVSANNIGSQQLKSLAIT
PSATGVAGGAIAVTGGGQTANIVIGAGQSAKVTASQLNGAIGGLSATASTEVKFGVNTAA
ITGSATGSANFTIKVGAGAAVSMVGVTDTAGLADQLKSNAAKLGISVNFNATTNNLEIKS
DTGENITLNSGDAGAIAGITVDAKDGAGNYSGAPVAAAAGAIQVTGAVSLNSTNSYSMSG
AGVTGLFGAATSASSAKTVVSNTDVTTAANAQNAVDVITQAISNIDSQRADLGAVQNRFD
STVANLRSISDNSTAARGVIQDVDFASESAQMTKQNTLQQASTAILAQANQLPAAVLKLL
Q