Protein Info for AO353_18625 in Pseudomonas fluorescens FW300-N2E3

Annotation: flagellar biosynthesis protein FlgL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR02550: flagellar hook-associated protein 3" amino acids 2 to 522 (521 residues), 253 bits, see alignment E=3.2e-79 PF00669: Flagellin_N" amino acids 3 to 139 (137 residues), 71.3 bits, see alignment E=4.3e-24

Best Hits

KEGG orthology group: K02397, flagellar hook-associated protein 3 FlgL (inferred from 57% identity to pba:PSEBR_a1465)

Predicted SEED Role

"Flagellar hook-associated protein FlgL" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VWE6 at UniProt or InterPro

Protein Sequence (524 amino acids)

>AO353_18625 flagellar biosynthesis protein FlgL (Pseudomonas fluorescens FW300-N2E3)
MRISTAQFYQNTAANYQRNFNNLLKSADQASSGVKLETAGDDPVGAARLLQLDQQSAMLG
QYTNNITALKTAQAQEESVLDSVNNVLQKVSELAVRAGGGSLNDDDRKSIAAELDQSEQQ
LLSLMNSKDANGNFLFSGASNDTAPFARNADGTYSYKGDQTQLQLKIGDSMSLGLNDSGW
SVFQQAVNASRSQTTMTAPAVDDGRVALSPGLVSSGPTFNSSFRSGEPYTLSFLSSTQFQ
IKDGAGNDVTAEATQGGTFDPNAKGGSDINFRGVTFGLDVTFQSGDTAINADAVIAGHSF
QLTTKPDTIASTRNPGNPSTALVSQSTVTNPADYAGFFPDGGAVLKFTSATTFDLYASPL
TSSSQAVSSGTVTAGVATAAGISFTMSGTPAANDQFDVSVNAHQTQNVLDTVSQLRQALE
TPVQGNPTAQRHLKESIASAIANLGNAKNQVDLTRGAIGARGNVMDLQSEQNQSAGLINA
STQSDIKNTDPAEVLTRLTLQQTMLQAAQLAFSKISQLGLFNKL