Protein Info for AO353_18620 in Pseudomonas fluorescens FW300-N2E3

Annotation: flagellar biosynthesis protein FlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 670 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR02492: flagellar hook-associated protein FlgK" amino acids 2 to 375 (374 residues), 263.8 bits, see alignment E=2e-82 PF00460: Flg_bb_rod" amino acids 4 to 33 (30 residues), 35.6 bits, see alignment (E = 1.1e-12) PF21158: flgK_1st_1" amino acids 338 to 420 (83 residues), 42.7 bits, see alignment E=6.9e-15 PF06429: Flg_bbr_C" amino acids 629 to 668 (40 residues), 25.6 bits, see alignment 1.5e-09

Best Hits

KEGG orthology group: K02396, flagellar hook-associated protein 1 FlgK (inferred from 58% identity to pfo:Pfl01_1506)

Predicted SEED Role

"Flagellar hook-associated protein FlgK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WIF4 at UniProt or InterPro

Protein Sequence (670 amino acids)

>AO353_18620 flagellar biosynthesis protein FlgK (Pseudomonas fluorescens FW300-N2E3)
MSLLNIGMSGLSAASSSLAVTGNNIANVDTAGYSRQQTVQSSKASIQYGNVFIGTGTTLA
DVRRVYNAYLDKQLQLTTSLSSDAQAYLSQVTQVDKLLSDSGTGITKVMQSFFSSLQTLS
STPNDAAARQALLTNSQSLSSRFNAMSDQLKQQGSYINAQMGSMVDQVNKLASTIATYNQ
KITAANGTGSAPNDLMDQRNETVRQLSQLVGTQVVERDGSYDIYMTGGQPLVMGSTANSL
QVSTDKSDPTRSSIIMNRGFSTLDITSAVTGGEMGGLLRYRDDVLAPATNALGRIAMVVS
DQVNRQLGQGLDQNGEFGAALFSNINSATAISQRSIASSNNNPASGNLNVTIQDTSKLST
SDYQVTFTSATGYSVKRLSDNTDMGSFTLGASPAPVIDGFSLSLNSAAGVSAGDNFKITP
TRSGAGDLSTVMTDTKRLSTSAPLTSTLAQGNKGTGSVSQPTLTSTLDIYNPVQSQDMQN
AIKTAMPVRMLMTSANAYEVLDAKGNSLGTGSIVPGQSNKLSIPVPYTDSLGAAQNFNFE
MTVSGNPVSGDSFNVSMTAASSTDNRNAQALLGLQSKATVGATTTSPGVSFSDAYGGLVS
EVGAKAKQGQMDGTANDTLLKGAAAARDSLSGVDLDEETGHLVKFQQYYSASSQIIKAAQ
EIFSTLINSL