Protein Info for AO353_18305 in Pseudomonas fluorescens FW300-N2E3

Annotation: RNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 PF00270: DEAD" amino acids 24 to 187 (164 residues), 151.6 bits, see alignment E=2.6e-48 PF04851: ResIII" amino acids 37 to 185 (149 residues), 33.2 bits, see alignment E=7.2e-12 PF00271: Helicase_C" amino acids 232 to 338 (107 residues), 101 bits, see alignment E=6.8e-33

Best Hits

Swiss-Prot: 40% identical to RHLE_ECOLI: ATP-dependent RNA helicase RhlE (rhlE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a1411)

MetaCyc: 40% identical to ATP-dependent RNA helicase RhlE (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase SrmB" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WEN2 at UniProt or InterPro

Protein Sequence (448 amino acids)

>AO353_18305 RNA helicase (Pseudomonas fluorescens FW300-N2E3)
VFSQFALHERLLKAVAELKFVEPTPVQAAAIPLALQGRDLRVTAQTGSGKTAAFVLPILN
RLIGPAKVRVSIKTLILLPTRELAQQTIKEVERFAQFTFIKSGLITGGEDFKVQAAMLRK
VPDILIGTPGRMIEQLNAGNLDLKEVEVLVLDEADRMLDMGFAEDVQRLVDECTNRQQTM
LFSATTGGSGLREMIAKVLNNPEHLQLNAVSQLNDTTRQQIITADHNQHKEQIVNWLLAN
ETYQKAIVFTNTRAMADRIYGRLVAQEYKAFVLHGEKDQKDRKLAIDRLKQGGVKILVAT
DVAARGLDVEGLDLVINFDMPRSGDEYVHRIGRTGRAGNDGLAISLICHGDWNLMSSIER
YLKQSFERRTIKEVKGTYGGPKKVKASGKAVGVKKKKVDAKGDKKKTGAKAPTKRKIANR
PKTDALSLVSKDGMAPLKRRKPQAPAAE