Protein Info for AO353_18300 in Pseudomonas fluorescens FW300-N2E3

Annotation: mechanosensitive ion channel protein MscS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 transmembrane" amino acids 26 to 50 (25 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 103 to 126 (24 residues), see Phobius details amino acids 144 to 164 (21 residues), see Phobius details amino acids 170 to 186 (17 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 188 to 256 (69 residues), 56.6 bits, see alignment E=2.3e-19 PF21082: MS_channel_3rd" amino acids 339 to 398 (60 residues), 21.6 bits, see alignment E=2.4e-08

Best Hits

KEGG orthology group: None (inferred from 91% identity to pba:PSEBR_a1410)

Predicted SEED Role

"Small-conductance mechanosensitive channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VRJ4 at UniProt or InterPro

Protein Sequence (432 amino acids)

>AO353_18300 mechanosensitive ion channel protein MscS (Pseudomonas fluorescens FW300-N2E3)
MDLKQLWLNTQDLWGALDQHPLLHSSLALMVLLVIALLLGRVARYVVLHAVKLLGRQPAM
QWFNDLFQNKVFHRLAQMTPSLVIQFGLNLVPELSKTIQNFLGNVALSFTILFLVLALSA
LLNAMLDIYARTEHARTRSIKGYVQLAKMVLFVFGAIIIVATLIDRSPLLLLSGLGAMSA
VILLVYKDTLLSFVASVQLTSNDMLRVGDWIEMPQVGADGDVVDITLHTVKVQNFDKTIV
SIPTWRLMSESFKNWRGMQQSGGRRIKRSLFIDASGVRFIHDDEEQKLTQVRLLTDYISR
KQAELKSWNEAQGNVAAMSANRRRMTNIGTFRAYALAYLKSHPEIQPNMTCMVRQMQTTA
QGVPLEIYCFTRTTAWADYERIQGDIFDYLLAVLPEFGLSLYQQPSGGDLRAGLLPAVLG
ASHIPEPEKRSM