Protein Info for AO353_18225 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent RNA helicase HrpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1303 TIGR01967: RNA helicase HrpA" amino acids 12 to 1300 (1289 residues), 2089.7 bits, see alignment E=0 PF00270: DEAD" amino acids 83 to 228 (146 residues), 31.5 bits, see alignment E=5.1e-11 PF13245: AAA_19" amino acids 83 to 198 (116 residues), 26.5 bits, see alignment E=2.3e-09 PF00271: Helicase_C" amino acids 287 to 410 (124 residues), 48.6 bits, see alignment E=3.1e-16 PF04408: HA2_N" amino acids 475 to 500 (26 residues), 28.9 bits, see alignment (E = 3.3e-10) PF21010: HA2_C" amino acids 501 to 557 (57 residues), 34.8 bits, see alignment (E = 6.9e-12) PF07717: OB_NTP_bind" amino acids 624 to 699 (76 residues), 70.2 bits, see alignment 4.9e-23 PF11898: DUF3418" amino acids 713 to 1300 (588 residues), 675.9 bits, see alignment E=1.9e-206

Best Hits

Predicted SEED Role

"ATP-dependent RNA helicase HrpA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VWU6 at UniProt or InterPro

Protein Sequence (1303 amino acids)

>AO353_18225 ATP-dependent RNA helicase HrpA (Pseudomonas fluorescens FW300-N2E3)
MTDESPSIDKLLKNLDHAMLADRHRLRRQLLELRKKPDEAKLALWVTRMQASCDQVLARR
ASLPLIRYDDSLPIAAKRDEIKEALLKHQVLIIAGETGSGKTTQLPKICLEIGRGQHGLI
GHTQPRRIAARSVASRVAEELATPLGALVGYQVRFEDQSDANTLIKLMTDGILLAETQND
RYLERYDTIIVDEAHERSLNIDFLLGYLKTLLPRRPDLKVIITSATIDLERFSKHFDDAP
IVEVSGRTFPVDTWYRPLTLEQDEEGNRVEDDLTVDQAILATLDEIAAYERSERRSPGDV
LVFLPGEREIRDAADMLRKAQLKHTEILPLYARLSPAEQQRIFQSHPGRRVVLATNVAET
SLTVPGIRYVIDSGTARISRYSYRAKVQRLPIEAISQASANQRKGRCGRVEPGICIRLYS
EEDFIGRPEFTDPEILRTNLAAVILQMLHLRLGEITDFPFIEPPDGKAISDGFNLLQELS
AVDRNSQLTPLGRQLARLPVDPRMGRMLLEAAKLGSLQEVLIVASAMSIQDPRERPPERQ
QAADQAHAQWKDVDSDFAGLVNLWRGFEEQRQALTASPLRNWCRKNFLNYLRLREWRDSH
RQLSLICRDMQLSLNKEPADYPKLHKAVLSGLLSQIGQKTEDGDYLGARQRRFWIHPSSG
LGKKRPQWLMTAELVETTKLYARMVAKIEPDWIEPLAGHLIKKNHFEPHWEKKRGQVVAY
EQITLFGLIVVGRRPVHYGPIDPVTSRELFIREALVRGEIQSKAKCLFANEQLLEQLDEL
EAKARRRDILADEETLFAFYDARLPAEIHQTATFDSWYRINSQKDPQLLIMREEDVLARE
ASEVTAAHYPDTLRLGDLTLPLSYHFEPNHPRDGVTLRVPAPLLPALPPERLEWLVPGVI
EAKCIALVRNLPKALRKNFVPVPDFVKAALQRVTFAEGSLPHALGRELLRMTGARVSDEA
WTEAAQQVESHLRMNLEIVDGQGKFLGEGRDLAELTARFAEASQAALAVPQTAKSQQPVE
AKVFAAVAEKTQQKIAGLSMTVYPALVEEAGSVKEGRFSTPAEAEYQHRRALQRLLMQQL
AEPAKFLRGKLPGLTELGLMYRELGRIDSLVEDILLASLDSCILDGEASLPRDGAGLASL
AERKRGAWTEHAERLAKLTLETLKLWHALQKRFKGKIDLAQAVALNDIKQQLSHLVYPGF
VRETPMQWLKELPRYLKAVEQRFEKLGAQVQKDRVWSGELSGLWTQYQTRANKHAQEGKR
DPQLELYRWWLEEYRVSLFAQQLGTKVPISDKRLSKQWTQVEP