Protein Info for AO353_18220 in Pseudomonas fluorescens FW300-N2E3

Annotation: isocitrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 PF20423: AceK_regulatory" amino acids 11 to 310 (300 residues), 340.9 bits, see alignment E=6.4e-106 PF06315: AceK_kinase" amino acids 312 to 566 (255 residues), 401.1 bits, see alignment E=2.2e-124

Best Hits

Swiss-Prot: 92% identical to ACEK_PSEPF: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 92% identity to pfo:Pfl01_1419)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WXN7 at UniProt or InterPro

Protein Sequence (573 amino acids)

>AO353_18220 isocitrate dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MPQPWPAADIAQMILDGFDDYREHFRQITDGARARFEKAQWQETQIASAARINLYEEKVG
ETVGCLHQSFADDVLMDVTCWPLVKSAYINLIDLRFDDELSETWYNSIFCGLFSHDLISD
GCMFIHTTRPSLHRARAAQTRIYKPHGQLSGMLASIFADYRFSEDYADLDRDLRRLEAQL
RENLPDWVCKDPDLSVELFSSVLYRNKGAYLVGRIYTADEQWPLVIPFLHREGRGIQIDA
LITDEAEVSIIFSFTRSYFMVDVPVPAEFIGFLKRILPGKHIAELYTSIGFYKHGKSEFY
RALINHLANTDDQFIMAPGVRGMVMSVFTLPGFNTVFKIIKDRFSPSKNVDRATVIEKYR
LVKSVDRVGRMADTQEFADFRFPLSKFDPACLAELLEVAASTVQVEGDTVLIRHCWTERR
MTPLNLYLENANDAQVREALEDYGLAIKQLAAANIFPGDMLLKNFGVTRHGRVVFYDYDE
ICFLTEANFRHIPQPRTPEDEMASEPWYSIGPLDVFPEEFPPFLFADSAQRRLFDQLHGE
LYNADYWKGLQAAIRAGKVIDVFPYRRKGLDNE