Protein Info for AO353_17440 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 PF00989: PAS" amino acids 14 to 95 (82 residues), 48.5 bits, see alignment E=2.9e-16 TIGR00229: PAS domain S-box protein" amino acids 14 to 122 (109 residues), 75.5 bits, see alignment E=4.2e-25 PF08448: PAS_4" amino acids 17 to 119 (103 residues), 48.6 bits, see alignment E=3.1e-16 PF13426: PAS_9" amino acids 20 to 116 (97 residues), 46.5 bits, see alignment E=1.3e-15 PF08447: PAS_3" amino acids 30 to 99 (70 residues), 49.9 bits, see alignment E=1.1e-16 PF01590: GAF" amino acids 139 to 274 (136 residues), 37.7 bits, see alignment E=1e-12 PF13185: GAF_2" amino acids 139 to 275 (137 residues), 42.6 bits, see alignment E=2.5e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 283 to 439 (157 residues), 154.7 bits, see alignment E=1.8e-49 PF00990: GGDEF" amino acids 287 to 439 (153 residues), 165.6 bits, see alignment E=2.9e-52

Best Hits

KEGG orthology group: None (inferred from 77% identity to pfo:Pfl01_1323)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJR6 at UniProt or InterPro

Protein Sequence (447 amino acids)

>AO353_17440 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3)
MTTSNSAPLASYIDLLLDVVCAVDSQGRFVFVSAASERVFGYTPDELIGQSMIEMVHPAD
RQRTLDAAREIMRGQPNPNFENRYLRKDGQVVHLLWSARWSEVDQLRIAVARDITERKQA
ESRQAALYAISEAAHAAEDLLALFKLVHQLIGEWLPALNFSVALYDEHCEQLSFPYHVDE
HESDPLCSVTGRLCTEVIETGQPVLVTPDQPFSMPGLFEVGNGQDSPCWLGVPLNSQKGT
IGALIVKSVPGGERYTERDKELLQYVCAQLAAAIERKQLHCRLQFMAQHDQLTRLPNREL
LRERLTAALGKARRNSGRMALLYVDLDHFKQVNDTYGHGVGDMLLQAVANRLQGCVRESD
TVARIGGDEFVVLLDSIQAAEDAARVAIKIRQVLGQPMRLESHTLNILPSIGIALYPEHG
SEEKQLFKHADEAMYAVKRLSGRLDRV