Protein Info for AO353_17140 in Pseudomonas fluorescens FW300-N2E3
Annotation: 2-hydroxy-3-oxopropionate reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 35% identical to GARR_ECOL6: 2-hydroxy-3-oxopropionate reductase (garR) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K00020, 3-hydroxyisobutyrate dehydrogenase [EC: 1.1.1.31] (inferred from 85% identity to pfl:PFL_1303)MetaCyc: 35% identical to tartronate semialdehyde reductase (Escherichia coli K-12 substr. MG1655)
2-hydroxy-3-oxopropionate reductase. [EC: 1.1.1.60]
Predicted SEED Role
"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)
MetaCyc Pathways
- superpathway of glycol metabolism and degradation (7/7 steps found)
- glycolate and glyoxylate degradation I (4/4 steps found)
- superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
- L-valine degradation I (6/8 steps found)
- D-glucarate degradation I (3/4 steps found)
- D-galactarate degradation I (3/4 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (12/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.31, 1.1.1.60
Use Curated BLAST to search for 1.1.1.31 or 1.1.1.60
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VVU1 at UniProt or InterPro
Protein Sequence (297 amino acids)
>AO353_17140 2-hydroxy-3-oxopropionate reductase (Pseudomonas fluorescens FW300-N2E3) MAALPSLAFAGIGLMGLPMCRRLLAAGYPLTVWNRNPDKCAPLVEAGARQVATPAELCQH ADLVMLCLANTEVVREVVFGAAGVASGAKTGQLLVDFSSLEPTATREMAAELVSRTGMGW VDAPVSGGVAGAEAASLAIMVGGESADVERVRLVLSSLGQRVTHMGAVGAGQVTKACNQM IVACNALVIAEVVALAERSGVEASLLAEALSGGFADSKPLQILAPQMAESRFEPIKWHVR TLLKDLDGAVKFSREQGSATPISGLAAQLMRLHAGQGYLEKDPATLIHLYREPDSKD