Protein Info for AO353_17055 in Pseudomonas fluorescens FW300-N2E3

Annotation: sodium:proton antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 61 to 80 (20 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 128 to 146 (19 residues), see Phobius details amino acids 156 to 177 (22 residues), see Phobius details amino acids 183 to 203 (21 residues), see Phobius details amino acids 212 to 239 (28 residues), see Phobius details amino acids 261 to 282 (22 residues), see Phobius details amino acids 294 to 319 (26 residues), see Phobius details amino acids 331 to 354 (24 residues), see Phobius details amino acids 366 to 386 (21 residues), see Phobius details PF06965: Na_H_antiport_1" amino acids 9 to 382 (374 residues), 495.3 bits, see alignment E=5.4e-153 TIGR00773: Na+/H+ antiporter NhaA" amino acids 10 to 381 (372 residues), 513.8 bits, see alignment E=1.4e-158

Best Hits

Swiss-Prot: 92% identical to NHAA_PSEPF: Na(+)/H(+) antiporter NhaA (nhaA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03313, Na+:H+ antiporter, NhaA family (inferred from 92% identity to pfo:Pfl01_1234)

MetaCyc: 55% identical to Na+:H+ antiporter NhaA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-129; TRANS-RXN-292

Predicted SEED Role

"Na+/H+ antiporter NhaA type" in subsystem Na(+) H(+) antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WLK6 at UniProt or InterPro

Protein Sequence (395 amino acids)

>AO353_17055 sodium:proton antiporter (Pseudomonas fluorescens FW300-N2E3)
LPLRSTFTRFFQLEAASGLLLIAAAALALIINNSPLSWLYNGLLDTPVVAQIGALKIAKP
LLLWINDGLMAMFFLLIGLEVKREILEGHLSKPSQIVLPGAAAIGGMVVPALIYWFLNKD
NPSALGGWAIPMATDIAFALGVLALLGKRVPVSLKLFLMTLAIIDDLGAIIVIAIFYSGA
LSTLSLMLAAACLVALVAMNRLGVVKLGPYMIIGLILWVCVLKSGVHATLAGVTLAFCIP
LRTKNAEPSPLLSLEHALHPWVAFGILPLFAFANAGVSLAGVNLESFTHHVPMGIAAGLL
LGKTVGVFGLTWLAVKIGIAALPAGANWGQVLGVAILCGIGFTMSLFVGSLAFVPGSSEY
AGMDRMGILTGSILAALIGYAVTAAASRKSINRAP