Protein Info for AO353_16855 in Pseudomonas fluorescens FW300-N2E3

Annotation: tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 TIGR00104: tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA" amino acids 2 to 142 (141 residues), 163 bits, see alignment E=2.6e-52 PF01980: TrmO" amino acids 21 to 138 (118 residues), 151.8 bits, see alignment E=9.2e-49 PF18389: TrmO_C" amino acids 155 to 216 (62 residues), 66.9 bits, see alignment E=1.4e-22

Best Hits

Swiss-Prot: 75% identical to RCSF1_PSEAE: Putative S-adenosylmethionine-dependent methyltransferase RcsF (rcsF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 93% identity to pfl:PFL_1242)

Predicted SEED Role

"COG1720: Uncharacterized conserved protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WWV1 at UniProt or InterPro

Protein Sequence (232 amino acids)

>AO353_16855 tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA (Pseudomonas fluorescens FW300-N2E3)
MTYSVSPIGFVRSCFKEKFAIPRQPQLAPAARGILELVAPFDQGDAVQGLEQVSHVWLLF
LFHQALEDKPRLKVRPPRLGGNKSMGVFATRATHRPNGIGQSVVKLDKVEANRLWISGID
LLDGTPILDIKPYVPYADIIPGASNEIASAAPQLIPVLWADSALQQAHTHAQRLEEPLVE
LIEQCLAQDPRPAYQTPAPEREYGAQFWDLDVRWHYPQAGLIRVLEVIPAKA