Protein Info for AO353_16450 in Pseudomonas fluorescens FW300-N2E3

Annotation: protein-PII uridylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 PF03445: DUF294" amino acids 34 to 128 (95 residues), 23.8 bits, see alignment E=8.9e-09 TIGR01693: protein-P-II uridylyltransferase" amino acids 37 to 885 (849 residues), 1100.5 bits, see alignment E=0 PF01909: NTP_transf_2" amino acids 68 to 116 (49 residues), 33.3 bits, see alignment 1.3e-11 PF08335: GlnD_UR_UTase" amino acids 185 to 322 (138 residues), 154.7 bits, see alignment E=3.9e-49 PF01966: HD" amino acids 487 to 573 (87 residues), 38.5 bits, see alignment E=3.2e-13 PF01842: ACT" amino acids 815 to 863 (49 residues), 31.2 bits, see alignment 3.7e-11

Best Hits

Swiss-Prot: 95% identical to GLND_PSEPF: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 95% identity to pfo:Pfl01_1099)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0A4 at UniProt or InterPro

Protein Sequence (900 amino acids)

>AO353_16450 protein-PII uridylyltransferase (Pseudomonas fluorescens FW300-N2E3)
MPQVDPELFDRGQFQAELALKASPIAAFKKAIRQAQEVLDARFRSGRDIRRLIEDRAWFV
DNILQQAWEQFNWSEDADIALVAVGGYGRGELHPYSDIDLLILLDNADHEVFRDSIERFL
TLLWDIGLEVGQSVRSVDECAEQARADLTIITNLMESRTIAGPEHLRQRMLDVTSTEHMW
PAKDFFLAKRAEQKARHHKYNDTEYNLEPNVKGSPGGLRDIQTILWVARRQYGTLNLRAL
AGEGFLVESENALLASSQEFLWKVRYALHMLAGRAEDRLLFDHQRSIAGLLGFEGDDAKH
AIENFMQQYYRVVMSIAQLSDLIIQHFEEVILAPEDEAPPQPINSRFQLHDGYIEAINTN
VFRRTPFAMLEIFVLMAQQPEIKGVRADTIRLLREHRHLIDDDFRNDIRNTSLFIELFKC
KIGIHRNLRRMNRYGILGRYLPEFGFIVGQMQHDLFHIYTVDAHTLNLIKHLRKLQYTQV
SEKFPLASKLMGKLPKPELIYLAGLYHDIGKGRHGDHSDIGAVDAEAFCQRHQLPLWDSR
LIVWLVQNHLVMSTTAQRKDLSDPQVIHDFAQIVGDETRLDYLYVLTVSDINATNPTLWN
SWRASLLRQLYTETKRALRRGLENPVDREEQIRQTQRAALDILVRGGTDPDDVEQLWSQL
GDDYFLRHTAGDVAWHTEAILQQPADGGPLVLIKETTQREFEGGTQIFIYAPDQHDFFAV
TVAAMDQLNLNIHDARVITSTSQFTLDTYIVLDTDGDSIGDNPARVKQIRDGLTEALRNP
DNYPTIIQRRVPRQLKHFAFAPQVTIHNDAQRQVTVLELSAPDRPGLLARIGTIFLEFDL
SLQNAKIATLGERVEDVFFITDASNHPLSDPQLCSRLQDAIVEQLSVSPEPSIELSRISI