Protein Info for AO353_16365 in Pseudomonas fluorescens FW300-N2E3

Annotation: multidrug transporter AcrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1021 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details transmembrane" amino acids 336 to 355 (20 residues), see Phobius details amino acids 362 to 382 (21 residues), see Phobius details amino acids 388 to 412 (25 residues), see Phobius details amino acids 427 to 451 (25 residues), see Phobius details amino acids 464 to 486 (23 residues), see Phobius details amino acids 532 to 550 (19 residues), see Phobius details amino acids 863 to 882 (20 residues), see Phobius details amino acids 889 to 909 (21 residues), see Phobius details amino acids 915 to 935 (21 residues), see Phobius details amino acids 963 to 983 (21 residues), see Phobius details amino acids 989 to 1013 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 6 to 1011 (1006 residues), 567.3 bits, see alignment E=6e-174 PF03176: MMPL" amino acids 849 to 1012 (164 residues), 27.3 bits, see alignment E=1.8e-10

Best Hits

KEGG orthology group: None (inferred from 86% identity to pmy:Pmen_3350)

Predicted SEED Role

"RND efflux transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H0A2 at UniProt or InterPro

Protein Sequence (1021 amino acids)

>AO353_16365 multidrug transporter AcrB (Pseudomonas fluorescens FW300-N2E3)
MRFNLSEWALHNRQIVLFLMILLAVVGALSYTKLGQSEDPPFTFKAMVIRTSWPGAAAQE
VSRQVTERIEKKLMETGEYERIVSFSRPGESQVTFMARDSMHSAQIPELWYQVRKKISDI
RQTLPPGIQGPFFNDEFGTTFGNIYALTGEGFDYAVLKDYADRIQIQLQRVKDVGKVDLL
GLQDEKIWIELSNVKLATLGLPLATVQQALEEQNAMSNAGFFETSSERLQLRVTGNFQTV
EQIRDFPIRVGDRTFRIGDVADVHRGFNDPPAPRMRFMGEDAIGLAVAMKDGGDILVLGK
ALELEFERIQKNLPAGMQLRKVSDQPAAVKSSVGEFVQVLVEALAIVLLVSFFSLGVRTG
MVVALAIPLVLAMTFACMYYLGIGLHKISLGALVLALGLLVDDAIIAVEMMAIKMEQGYD
RLKAASFAWTSTAFPMLTGTLITAAGFLPIATAQSGTGEYTRSIFQVVTIALLASWVAAV
MFVPYLGEKLLPDLAKIHAAKHGAGQPDPYGTPFYQRVRRLVEWCVRRRKTVIALTILLF
VASVLLFRFVPQQFFPASGRLELMVDLKLAEGASLSNTNEEVKRLEAMLKERAGIDNYVA
YVGTGSPRFYLPLDQQLPAASFAQFVVLAKTIEERESLRTWLIETLNEQFPALRSRVTRL
ENGPPVGYPVQFRVTGEHIEEVRALARKVAAKVRENPHVVNVHLDWEEPSKVVYLNVDQD
RARALGVSTANLSRFLQSSLTGSSVSQYREDNELIEILLRGTLHERTELSRLSSLAVPTD
NGKSVALSQVATLEYGFEEGIIWHRNRLPNVTIRADIYDKEQPATLVKQILPTLEPIRAE
LPDGYLLDVGGTVEDSTRGQDSVNAGVPLFIVVVLTLLMLQLRSFSRTAMVFLTAPLGLI
GVTLFLMVFRQPFGFVAMLGTIALSGMIMRNSVILVDQIEQDILAGLKPWQAIIEATVRR
FRPIVLTALAAVLAMIPLSRSVFFGPMAVAIMGGLIVATALTLLFLPALYAAWFRVKKEP
V