Protein Info for AO353_16295 in Pseudomonas fluorescens FW300-N2E3

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF12146: Hydrolase_4" amino acids 64 to 168 (105 residues), 56.8 bits, see alignment E=4.1e-19 PF00561: Abhydrolase_1" amino acids 67 to 175 (109 residues), 46.7 bits, see alignment E=6.7e-16 PF12697: Abhydrolase_6" amino acids 68 to 229 (162 residues), 36 bits, see alignment E=2.5e-12 PF02129: Peptidase_S15" amino acids 88 to 177 (90 residues), 24.9 bits, see alignment E=3.1e-09

Best Hits

KEGG orthology group: K06889, (no description) (inferred from 82% identity to pfl:PFL_1142)

Predicted SEED Role

"Hydrolase of the alpha/beta superfamily in cluster with COG2110"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WNR5 at UniProt or InterPro

Protein Sequence (304 amino acids)

>AO353_16295 alpha/beta hydrolase (Pseudomonas fluorescens FW300-N2E3)
MKVLGILCLLLTLGGCSSLLFYPDPGLPFTPQRAGLEYRDITLTSADGVKLNGWWLPAKK
GVAVKGTVLHLHGNGGNLAGHLGASWWLPKQGYQVLLVDYRGYGLSQGKPGLPAIYQDID
AAFKWLDQAPEVQGKPLIVLGQSLGGALVVHYLAEHPERQPRLKALVLDSVPASYRDVGR
FALSSSWMTWPFQVPLSWLVPDADSAIYAMPQLNRVPKLIFHSIDDPLVPLSNGIRLYQA
APAPKVLQLTRGGHVQTFSVPIWRTVMLRYLDDPQHFNGLRRLGETPNYPAPPNSEVEPP
ESPQ