Protein Info for AO353_16215 in Pseudomonas fluorescens FW300-N2E3

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 323 (320 residues), 401.1 bits, E=4.2e-124
  • amino acids 554 to 700 (147 residues), 27.8 bits, E=1.8e-10
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 7 to 768 (762 residues), 1117.7 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 441 to 763 (323 residues), 521.3 bits, E=1.1e-160

Best Hits

Swiss-Prot: 82% identical to METE_PSESM: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 89% identity to pfo:Pfl01_4545)

MetaCyc: 63% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See A0A0N9WL62 at UniProt or InterPro

Protein Sequence (770 amino acids)

>AO353_16215 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (Pseudomonas fluorescens FW300-N2E3)
MALAHTLGFPRIGADRELKKALESYWKGDLDQAGLQSVGRELRARHWQVQKDAGIDLLPV
GDFAWYDQVLTHSLTFGVIPERFDSAKDAQGLPTLDTLFAMARGASAKCCDGESAKTHHA
QELTKWFDTNYHYLVPEFSADQPFKLSWEQLFDEVDEAHALGHRVKPVIIGPLTYLWLGK
TKGNDFDKLELLERLLPLYGEILNRLKAQGVEWVQIDEPILTLDLPQAWKNAFERAYHIL
QYSPLKKLVATYFSGLEDNLGLAVSLPVDGLHIDAVRAPEQLGQVLDRLPTYKVLSVGLV
NGRNVWRCELEQALAQLKPAQERYGDNLWVSSSCSLLHSPVDLDREDTLDAELKSWLAFA
VQKCAEIAVLRDALNDPQAPKVQAALAQNRAVQASRAQSPRIHKAEVQARIRAIRDTDSQ
RHSVFAKRIELQRARLNLPAFPTTTIGSFPQTASIRLARQSFKQGKLSANDYTDAMHSEI
RHAVQIQERLGLDVLVHGEAERNDMVEYFAEQLDGYLFTRFGWVQSYGSRCVKPAIIYGD
LSRPKAMTVDWITYAQSLTDKVMKGMLTGPVTMLMWSFPREDVSRKVQAQQLALALRDEV
VDLEQAGIKIVQIDEAAFREGLPLRRAQWQEYLDWAVQAFRLSASGVRDETQIHTHMCYS
EFNDVIESIAAMDADVITIETSRSDMELLEAFKAFDYPNDIGPGVYDIHSPRVPDTTEMV
KLMSKAVQRIPAERLWVNPDCGLKTRAWPETEAALVNMVAAARQLRNQLA