Protein Info for AO353_16160 in Pseudomonas fluorescens FW300-N2E3
Annotation: glutamate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 95% identity to pfo:Pfl01_4559)Predicted SEED Role
"Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.7.1)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis V (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- GABA shunt II (2/4 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.7.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N7H097 at UniProt or InterPro
Protein Sequence (538 amino acids)
>AO353_16160 glutamate synthase (Pseudomonas fluorescens FW300-N2E3) MSLSLLSRYAFFAVCVIFTLASLPFLEYDWLWPISIVTGLLSLLGIFDLLQSPHAVRRNY PILGNIRYLVEGIRPEIRQYLLESDSDALPFSRAQRSLVYSRAKNESADKPFGTLIDVYQ SGFEFIGHSMRPAPVSDPSSFRVAVGGPQCTKPYSASVFNISAMSFGSLSANAIRALNQG AKLGNFAHDTGEGSISPYHREHGGDLTWELGSGYFGCRTADGHFDPERFAAQAQDPQVRM IEIKMSQGAKPGHGGILPKHKVTQEIADTRGVPMGQDCVSPSRHSAFSTPIEMMHFIKQL RELSGGKPVGFKFCLGHPWEFMGIAKAMLETGILPDFIVVDGKEGGTGAAPVEFTDHIGV PMREGLLFVHNTLVGLNLRDKIKLGASGKIVSAFDIASVLAIGADWANSARGFMFAIGCI QSQSCHTNKCPTGVATQDTLRQRALVVPDKAQRVFNFHRSTLKALAEMLAAAGLEHPSQL SAKHLVRRMSATEIKLFSQLHVFLKPGELLTGEVNGEFYSRMWQMARADSFEANEVAA