Protein Info for AO353_16135 in Pseudomonas fluorescens FW300-N2E3

Annotation: bactoprenol glucosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 transmembrane" amino acids 228 to 249 (22 residues), see Phobius details amino acids 261 to 286 (26 residues), see Phobius details PF00535: Glycos_transf_2" amino acids 4 to 165 (162 residues), 102.4 bits, see alignment E=1.4e-33

Best Hits

Swiss-Prot: 64% identical to GTRB_BPP22: Bactoprenol glucosyl transferase (gtrB) from Salmonella phage P22

KEGG orthology group: None (inferred from 67% identity to dda:Dd703_0125)

MetaCyc: 62% identical to CPS-53 (KpLE1) prophage; bactoprenol glucosyl transferase (Escherichia coli K-12 substr. MG1655)
Phosphopolyprenol glucosyltransferase. [EC: 2.4.1.78]

Predicted SEED Role

"Glycosyltransferase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.78

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJX2 at UniProt or InterPro

Protein Sequence (312 amino acids)

>AO353_16135 bactoprenol glucosyl transferase (Pseudomonas fluorescens FW300-N2E3)
VKISLIVPVFNEEQAINLFYQAVRQELSIADCDVEIVFINDGSSDQTAAQAKAIALADEH
VLVINFSRNFGKEPALFAGLEYATGDAVIPMDVDLQDPINVIPLLVREWQNGADVVLAKR
HDRTADSYMKRHSAALFYHLLNRIAYTRIEENVGDFRLMDRKVVNVIRTLPEHRLFMKGI
LSWAGFNTAVVEYERAERVAGTTKFNLWKLWNLALEGITSFSTVPLQLWTYVGSAISLFA
ILYAIYMVLDKIFFGNNVPGYPSLMTAILFLGGVQLIGIGILGEYVGRIYIESKHRPRYV
VKDIVGGKGVPK