Protein Info for AO353_16120 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): D-alanine and L-alanine transporter
Rationale: Specific phenotype on D-alanine as the carbon source. Also important for utilizing L-alanine as a carbon source. Both of these phenotypes are conserved. For D-alanine as a nitrogen source, there is a mild phenotype, and it may be partly redundant with another amino acid transporter (AO353_04615:04600). Sometimes annotated as a glycine transporter, but neither it nor its orthologs are important on glycine.
Original annotation: D-alanine/D-serine/glycine permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 transmembrane" amino acids 27 to 47 (21 residues), see Phobius details amino acids 54 to 73 (20 residues), see Phobius details amino acids 107 to 129 (23 residues), see Phobius details amino acids 135 to 153 (19 residues), see Phobius details amino acids 164 to 187 (24 residues), see Phobius details amino acids 208 to 230 (23 residues), see Phobius details amino acids 250 to 270 (21 residues), see Phobius details amino acids 291 to 313 (23 residues), see Phobius details amino acids 344 to 361 (18 residues), see Phobius details amino acids 367 to 388 (22 residues), see Phobius details amino acids 409 to 431 (23 residues), see Phobius details amino acids 437 to 456 (20 residues), see Phobius details PF00324: AA_permease" amino acids 26 to 449 (424 residues), 405.3 bits, see alignment E=3.6e-125 PF13520: AA_permease_2" amino acids 29 to 451 (423 residues), 138.9 bits, see alignment E=2.4e-44

Best Hits

Swiss-Prot: 67% identical to YTNA_BACSU: Uncharacterized amino acid permease YtnA (ytnA) from Bacillus subtilis (strain 168)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 96% identity to pba:PSEBR_a4518)

MetaCyc: 52% identical to threonine/serine:H+ symporter ThrP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-71; TRANS-RXN-72

Predicted SEED Role

"D-serine/D-alanine/glycine transporter" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH98 at UniProt or InterPro

Protein Sequence (472 amino acids)

>AO353_16120 D-alanine and L-alanine transporter (Pseudomonas fluorescens FW300-N2E3)
MTVGNHLPHGETAQGGPLKRELGERHIRLMALGACIGVGLFLGSAKAIEMAGPAIMLSYI
IGGLAILVIMRALGEMAVHNPVAGSFSRYAQDYLGPLAGFLTGWNYWFLWLVTCVAEITA
VAVYMGIWFPEVPRWIWALAALVSMGSINLIAVKAFGEFEFWFALIKIVTIIAMVVGGVG
VIAFGFGNDGVALGISNLWSHGGFMPNGVQGVLMSLQMVMFAYLGVEMIGLTAGEAKNPQ
KTIPNAIGSVFWRILLFYVGALFVILSIYPWNEIGTQGSPFVMTFERLGIKTAAGIINFV
VITAALSSCNGGIFSTGRMLYSLAQNGQAPAGFAKTSNGVPRRALLLSIGALLLGVLLNY
LVPEKVFVWVTAIATFGAIWTWVMILLAQLKFRKGLSPAERAALKYRMWLYPVSSYLALA
FLVMVVGLMAYFPDTRVALYVGPAFLVLLTVLFYVFKLQPTGVPQAAVRTAS