Protein Info for AO353_15995 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): trehalose-specific PTS system, I, HPr, and IIA components
Rationale: Specific phenotype on trehalose. The IIB/IIC components are provided by AO353_15980. 45% similar to a NAG PTS system (PA3760, PMC:PMC2542419)
Original annotation: PTS mannose transporter subunit IIC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 844 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 8 to 129 (122 residues), 140.8 bits, see alignment E=4.9e-45 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 9 to 127 (119 residues), 126.7 bits, see alignment E=7.4e-41 PF00381: PTS-HPr" amino acids 171 to 250 (80 residues), 82.1 bits, see alignment E=6.2e-27 TIGR01003: phosphocarrier, HPr family" amino acids 171 to 249 (79 residues), 66.5 bits, see alignment E=2.5e-22 PF05524: PEP-utilisers_N" amino acids 278 to 394 (117 residues), 78.7 bits, see alignment E=1e-25 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 278 to 826 (549 residues), 490.2 bits, see alignment E=1.1e-150 PF00391: PEP-utilizers" amino acids 419 to 492 (74 residues), 70.6 bits, see alignment E=1.8e-23 PF02896: PEP-utilizers_C" amino acids 525 to 804 (280 residues), 335.5 bits, see alignment E=6.4e-104

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 75% identity to pfs:PFLU5037)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH78 at UniProt or InterPro

Protein Sequence (844 amino acids)

>AO353_15995 trehalose-specific PTS system, I, HPr, and IIA components (Pseudomonas fluorescens FW300-N2E3)
MTLTQPLQLLAPLSGVLMPLDHVPDPVFASRVIGDGLCIDPTSQVLCAPLAGVVSNLQHS
GHAISITDDSGVQVLLHIGLDTVNLKGQGFSALVEQGQRVEAGQPLIEFDADYVALHARS
LLTLMLVVSGEPFSLLTPDSGLVACAQPVLRLSLGDPRTVVAQEEGEALFSKPVHLPNPN
GLHARPAAVFAQAAKGFAASICLHKQQDSANAKSLVAIMALQTVHGDALQVSAVGEDAEL
AISTLAQLLADGCGEAVTPVAVVAPVVEAQEVSTKLLRGVCASAGSAFGYVVQVAERTLE
MPEFAADQQLERESLERALMHATQALQRLRDNAAGEAQADIFKAHQELLEDPSLLEQAQA
LIAEGKSAAFAWNSATEATATLFKSLGSTLLAERALDLMDVGQRVLKLILGVPDGVWELP
DQAILIAEQLTPSQTAALDTGKVLGFATVGGGATSHVAILARALGLPAVCGLPLQVLSLA
SGTRVLLDADKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAARAAVTRDGHHFEVTA
NVASLAETEQAMSLGAEGIGLLRSEFLYQQRSVAPSHDEQAGTYSAIARALGPQRNLVVR
TLDVGGDKPLAYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAILSSAGLARLHIMLPM
VSQLSELRLARLMLEEEALALGLRELPKLGIMIEVPAAALMADLFAPEVDFFSIGTNDLT
QYTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLG
LGVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVLGLESAEQVREALSVQQQAMVETSQ
VLES