Protein Info for AO353_15980 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): trehalose PTS system, EII-BC components TreB
Rationale: Specifically important for trehalose utilization.
Original annotation: trehalose permease IIC protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 114 to 136 (23 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 189 to 207 (19 residues), see Phobius details amino acids 237 to 254 (18 residues), see Phobius details amino acids 266 to 288 (23 residues), see Phobius details amino acids 294 to 313 (20 residues), see Phobius details amino acids 315 to 336 (22 residues), see Phobius details amino acids 348 to 370 (23 residues), see Phobius details amino acids 405 to 423 (19 residues), see Phobius details amino acids 442 to 466 (25 residues), see Phobius details TIGR01992: PTS system, trehalose-specific IIBC component" amino acids 8 to 469 (462 residues), 714.3 bits, see alignment E=6.9e-219 PF00367: PTS_EIIB" amino acids 11 to 43 (33 residues), 42.2 bits, see alignment (E = 4.4e-15) TIGR00826: PTS system, glucose-like IIB component" amino acids 30 to 113 (84 residues), 50.9 bits, see alignment E=1.5e-17 PF02378: PTS_EIIC" amino acids 112 to 408 (297 residues), 161.4 bits, see alignment E=3.1e-51

Best Hits

Swiss-Prot: 52% identical to PTTBC_BACSU: PTS system trehalose-specific EIIBC component (treP) from Bacillus subtilis (strain 168)

KEGG orthology group: K02818, PTS system, trehalose-specific IIB component [EC: 2.7.1.69] K02819, PTS system, trehalose-specific IIC component (inferred from 93% identity to pfl:PFL_4934)

MetaCyc: 47% identical to Enzyme IIscr (Staphylococcus xylosus)
SUCROSEPHOSPHO-RXN [EC: 2.7.1.211]

Predicted SEED Role

"PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69)" in subsystem Trehalose Uptake and Utilization (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.211 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDQ5 at UniProt or InterPro

Protein Sequence (480 amino acids)

>AO353_15980 trehalose PTS system, EII-BC components TreB (Pseudomonas fluorescens FW300-N2E3)
MSHDYPTIASELLHSLGGADNLEQAAHCVTRLRLALKDPQRVDSATLNQIDLVKGSFFTG
GLFQVVIGPGEVEKVYAALRQQTGLAASTIADVKQKGADKTNAMQRLVRVFSDVFMPILP
ALIIAGLLMGVNNLIGAKGMFIAGKTLLEAYPTLDGVWSLINLMANTSFVFLPALVGWSA
AKRFGGSEILGIVLGLMLVHPDLLNAWNYGKAVAGLDGQSLPYFDIFGWFKIEKVGYQGQ
ILPILMAAYVMSVIEKWLRARVPNAIQLLVVPITTIVVTGVLALAVIGPVTRHLGILITE
GVVTLFDLAPMVGGAIFGLLYAPLVITGMHHMFLAVDLQLISTQGGTFIWPMIVMSNLAQ
GSAALGVFYMTRSARDKSMASTSAISAYFGITEPAMFGVNLRYKFPFYAALSGSALGCMF
LSLNKVQASAIGVGGLPGFISIIPQFIPMFIVGMVIAMVVPFVLTCGMSMKIVRAGYRVA