Protein Info for AO353_15575 in Pseudomonas fluorescens FW300-N2E3
Annotation: pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 41% identical to ODPA_STAAC: Pyruvate dehydrogenase E1 component subunit alpha (pdhA) from Staphylococcus aureus (strain COL)
KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 85% identity to avn:Avin_10770)Predicted SEED Role
"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.1, 1.2.4.4
Use Curated BLAST to search for 1.2.4.1 or 1.2.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N7H084 at UniProt or InterPro
Protein Sequence (365 amino acids)
>AO353_15575 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha (Pseudomonas fluorescens FW300-N2E3) MPRNKVDLPYTRYLSPDGQALSELPAWADDFNLLTRLYRQMVLTRLFDQKAVALQRTGRI GTYAPTLGQEAIGVAVGSLMHPEDVLIPYYRDTGVQLMRGVRMEEILLYWGGDERGSDFA EPAAVEDFPICVPIATQALHACGVASAFKIRGEHRVAVTTCGDGATSKGDFLEALNVAGA WQLPVVFVINNNQWAISVPRRIQCAAPTLAQKAIGAGFHGEQVDGNDILAVYDRVQIALE RARHGKGPVLLECVSYRLGDHTTADDATRYRPAEEVKQAWLEEPVKRLQRYLAGQGVWDE GREQALIAECQALVQGAVDNFDAAGQQSAESVIDHVYAQWPAALAEQREWLLERAARRAG GAAHE