Protein Info for AO353_15180 in Pseudomonas fluorescens FW300-N2E3

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 PF00579: tRNA-synt_1b" amino acids 3 to 282 (280 residues), 172.8 bits, see alignment E=5.5e-55 TIGR00233: tryptophan--tRNA ligase" amino acids 4 to 335 (332 residues), 321.4 bits, see alignment E=3.5e-100

Best Hits

Swiss-Prot: 84% identical to SYW_PSESM: Tryptophan--tRNA ligase (trpS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 96% identity to pfo:Pfl01_4699)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VUU2 at UniProt or InterPro

Protein Sequence (451 amino acids)

>AO353_15180 tryptophan--tRNA ligase (Pseudomonas fluorescens FW300-N2E3)
MTTRTRILTGITTTGTPHLGNYAGAIRPAILASRDSNADSFYFLADYHALIKCDDPLRIQ
RSRLEIAATWLAGGLDVDRVTFYRQSDIPEIPELTWLLTCVAAKGLLNRAHAYKASVDKN
VEAGEDPDAGITMGLYSYPVLMAADILMFNAHKVPVGRDQIQHVEMARDIGQRFNHLFGQ
GKEFFTMPEALIEESVATLPGLDGRKMSKSYDNTIPLFSSAKEMKDAISRIVTDSRAPGE
AKDPDNSHLFTLFQAFATPAQADEFRSELLQGLGWGEAKNRLFQLLDNELGESRERYYQL
IDRPADLEDILQHGAAKARAVATPFLNELREAVGLRSFVKQTQVAATTKKKAAKAARFVS
FREDDGSFRFRLLAADGEQLLLSRNFADGKTAGQVTKQLQSGQALDVRSEDLSFSVWLEG
ECVADSPAFADSTARDVAIDALRVALTPVQE