Protein Info for AO353_14855 in Pseudomonas fluorescens FW300-N2E3

Annotation: uracil phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 TIGR01091: uracil phosphoribosyltransferase" amino acids 3 to 208 (206 residues), 294.6 bits, see alignment E=1.9e-92 PF14681: UPRTase" amino acids 7 to 207 (201 residues), 227.9 bits, see alignment E=9.4e-72 PF00156: Pribosyltran" amino acids 54 to 171 (118 residues), 53.7 bits, see alignment E=1.6e-18

Best Hits

Swiss-Prot: 97% identical to UPP_PSEPF: Uracil phosphoribosyltransferase (upp) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00761, uracil phosphoribosyltransferase [EC: 2.4.2.9] (inferred from 97% identity to pfo:Pfl01_4728)

MetaCyc: 73% identical to uracil phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Uracil phosphoribosyltransferase. [EC: 2.4.2.9]

Predicted SEED Role

"Uracil phosphoribosyltransferase (EC 2.4.2.9)" in subsystem De Novo Pyrimidine Synthesis or LMPTP YwlE cluster (EC 2.4.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.9

Use Curated BLAST to search for 2.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VUP4 at UniProt or InterPro

Protein Sequence (212 amino acids)

>AO353_14855 uracil phosphoribosyltransferase (Pseudomonas fluorescens FW300-N2E3)
MPIREIRHPLIRHKLGLMRRADISTKNFRELAQEVGALLTYEATKDLPLETYDIEGWCGT
VSVEKIAGKKITVVPILRAGIGMLEGVLSLIPGAKVSAVGVARNEETLEAHTYLEKLVPE
IDERLAMIIDPMLATGGSMVATIDLLKKAGCKDIRAMVLVAAPEGIAAVEKAHPDVTIYT
ASIDQKLNEHGYIIPGLGDAGDKIFGTKQKDA