Protein Info for AO353_14475 in Pseudomonas fluorescens FW300-N2E3

Annotation: penicillin-binding protein 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 774 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 24 to 748 (725 residues), 1021.7 bits, see alignment E=0 PF17757: UvrB_inter" amino acids 67 to 143 (77 residues), 30.8 bits, see alignment E=5.3e-11 PF14814: UB2H" amino acids 69 to 152 (84 residues), 85.5 bits, see alignment E=4.1e-28 PF00912: Transgly" amino acids 166 to 334 (169 residues), 192.5 bits, see alignment E=9e-61 PF00905: Transpeptidase" amino acids 429 to 668 (240 residues), 64.5 bits, see alignment E=2e-21

Best Hits

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 94% identity to pfo:Pfl01_4792)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WVR3 at UniProt or InterPro

Protein Sequence (774 amino acids)

>AO353_14475 penicillin-binding protein 1B (Pseudomonas fluorescens FW300-N2E3)
MTRTRSPRTPKKPPSRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPA
KVYARPLELFVGQKLSKDDFLTELDALGYRRESVANGPGAASVNGNTVDLNTRGFQFYEG
LEQAQPVRVRFSGDYVAELSSTNGSKLSVVRLEPLLIGGLYPKNLEDRILIKIDQVPPYL
LDTLVAVEDRDFYHHFGVSPKSIMRAVWVNTSAGQMRQGGSTLTQQLVKNFYLTNERSLS
RKLTEAMMAMLLELHYKKPDILEAYLNEVFIGQDGQRAVHGFGLASQFFFSQPLSELKLH
QVALLVGMVKGPSYYNPRRYPERALERRNLVLDLLEQQGVATAEQVAAAKKMPLGVTKRG
SLADSSFPGFLDLVKRQLREDYRDEDLTEEGLRIFTSFDPILQMKAEASVNDTFKRLAGR
KGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKP
SQYTLTSWLSDESFSVKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTARLGLEVGV
PNVLKTLGRLGITREFPAFPSMLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTAE
GQPLKRYPFQIQQRFDPASIYLIQSAMQRVMREGTGSSVYNVLPKTLTLAGKTGTSNDSR
DSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNIVQA
WVDSRTGQGSEASCPGAVQMPYIRGSEPPPGAACGGSGPASGESVMDWVKGWMN