Protein Info for AO353_14330 in Pseudomonas fluorescens FW300-N2E3

Annotation: general secretion pathway protein GspE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR02538: type IV-A pilus assembly ATPase PilB" amino acids 8 to 566 (559 residues), 848.4 bits, see alignment E=1.3e-259 PF05157: MshEN" amino acids 42 to 146 (105 residues), 72.3 bits, see alignment E=3.6e-24 PF00437: T2SSE" amino acids 190 to 457 (268 residues), 364 bits, see alignment E=4.2e-113

Best Hits

Swiss-Prot: 78% identical to PILB_PSEAE: Type 4 fimbrial assembly protein PilB (pilB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02652, type IV pilus assembly protein PilB (inferred from 91% identity to pba:PSEBR_a4826)

Predicted SEED Role

"Type IV fimbrial assembly, ATPase PilB" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W6F3 at UniProt or InterPro

Protein Sequence (566 amino acids)

>AO353_14330 general secretion pathway protein GspE (Pseudomonas fluorescens FW300-N2E3)
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNRVSLVSYLVQNKMVSSRQVAEIASEH
FGVAFLDLNCLDKDSQPKDLVSEKLIRQHHALPLWRRGNKLFVGISDPSNQQAINDIQFS
TGLTTEAILVEDDKLSDAIEKFFDSHSTGLEDMADIDLGGPDIEAVDDQRQDAVTGQDSD
DAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPTQLANRIAARL
KVMASLDISERRKPQDGRLKMHLSKSKSIDFRVNTLPTLWGEKIVIRILDPSSAQMGIDA
LGYEPDQKDLYMAALKQPQGLILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEIN
MEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHLVLSTL
HTNSAAETLTRLHNMGIPGFNIATAVHLIIAQRLARKLCSHCKKNLSIPDETLIKEGFPR
ERIGSFTIYEPVGCDLCNNGYKGRVGIYEVVKNTPQLQRLIMAEGNSLEIDTQMRKDGFN
DLRTSGLLKAMQGITSLEEINRVTKD