Protein Info for AO353_14255 in Pseudomonas fluorescens FW300-N2E3

Annotation: NADH dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07992: Pyr_redox_2
  • amino acids 4 to 337 (334 residues), 181.1 bits, E=4.9e-57
• PF00070: Pyr_redox
  • amino acids 170 to 253 (84 residues), 40.6 bits, E=4.5e-14

Best Hits

Swiss-Prot: 56% identical to DHNA_ECOLI: NADH dehydrogenase (ndh) from Escherichia coli (strain K12)

KEGG orthology group: K03885, NADH dehydrogenase [EC: 1.6.99.3] (inferred from 95% identity to pfo:Pfl01_4831)

MetaCyc: 56% identical to NADH:quinone oxidoreductase II (Escherichia coli K-12 substr. MG1655)
RXN0-5330 [EC: 1.6.5.9]; 1.6.5.9 [EC: 1.6.5.9]; 1.16.1.- [EC: 1.6.5.9]

Predicted SEED Role

"NADH dehydrogenase (EC 1.6.99.3)" in subsystem Carboxysome or Respiratory dehydrogenases 1 (EC 1.6.99.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• NADH to cytochrome bo oxidase electron transfer II (2/2 steps found)
• NADH to nitrate electron transfer (2/2 steps found)
• mitochondrial NADPH production (yeast) (4/5 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration II (cytochrome c) (yeast) (3/4 steps found)
• NADH to cytochrome aa₃ oxidase electron transfer (1/2 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NADH to cytochrome bd oxidase electron transfer II (1/2 steps found)
• nitrate reduction VIIIb (dissimilatory) (1/2 steps found)
• Fe(II) oxidation (3/6 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.5.9 or 1.6.99.3

Sequence Analysis Tools

See A0A0N9WKC3 at UniProt or InterPro

Protein Sequence (432 amino acids)

>AO353_14255 NADH dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MTHRIVIVGGGAGGLELATRLGKTLGKRGTASVMLVDANLTHIWKPLLHEVAAGSLNSSE
DELNYVAQAKWNHFEFQLGRMSGLDRAQKKIQLAATYDENGRELVPAREVAYDSLVISVG
STTNDFGTQGAAQHCLFLDTRKQAERFHQQLLNHYLRAHAGQSDAVQQISVAIVGAGATG
VELAAELHNAAHELAAYGLDRILPENMHITLIEAGPRVLPALPERIGGPVHKTLEKLGVT
VLTNAAVSEVTADALITASGQEIKASLKVWAAGIRAPGFLKDIDGLETNRINQLQVLPTL
QTTRDENIFAFGDCAACPQPGTDRNVPPRAQAAHQQASLLAKSLKLRIEGKPLPTYKYTD
YGSLISLSRFSAVGNLMGNLTGSVMLEGWLARMFYVSLYRMHQVALYGPFRTAMLMLGSR
IGRGTEPRLKLH