Protein Info for AO353_14230 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent chaperone ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 853 (848 residues), 1447.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 42.3 bits, see alignment 4.9e-14 amino acids 94 to 145 (52 residues), 43 bits, see alignment 2.8e-14 PF00004: AAA" amino acids 203 to 335 (133 residues), 50.4 bits, see alignment E=2.3e-16 amino acids 602 to 720 (119 residues), 36 bits, see alignment E=6.4e-12 PF17871: AAA_lid_9" amino acids 342 to 445 (104 residues), 121 bits, see alignment E=1.3e-38 PF07724: AAA_2" amino acids 596 to 758 (163 residues), 236.3 bits, see alignment E=1.3e-73 PF00158: Sigma54_activat" amino acids 600 to 708 (109 residues), 22.9 bits, see alignment E=4.2e-08 PF07728: AAA_5" amino acids 601 to 722 (122 residues), 49.5 bits, see alignment E=3.3e-16 PF10431: ClpB_D2-small" amino acids 764 to 843 (80 residues), 88.7 bits, see alignment E=1.5e-28

Best Hits

Swiss-Prot: 94% identical to CLPB_PSESM: Chaperone protein ClpB (clpB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 73% identity to abo:ABO_0479)

MetaCyc: 70% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H054 at UniProt or InterPro

Protein Sequence (854 amino acids)

>AO353_14230 ATP-dependent chaperone ClpB (Pseudomonas fluorescens FW300-N2E3)
MRIDRLTSKLQLALSDAQSLAVGLDHPGIEPAHLMQALLEQQGGSIKPLLMQVGFDINSL
RKELSKELDRLPKIQNPTGDVNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENS
KLGKLLLGQGVSKKALENAINNLRGGEAVNDANVEESRQALDKYTIDLTKRAEEGKLDPV
IGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLKSKRLLSL
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKAEGSMDAGNMLK
PALARGELHCVGATTLNEYRQYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVH
HKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASRIRMEIDSKPEVLDRLERRLI
QLKVESQALKKESDEAAKKRLEKLQEEIVRHEREYSDLEEIWNSEKAEVQGSAQIQQKIE
QSRQELEAARRKGDLNRMAELQYGVIPDLERSLQMVDQHGKSENQLLRSKVTEEEIAEVV
SKWTGIPVSKMLEGERDKLLKMESLLHQRVIGQNEAVVAVANAVRRSRAGLSDPNRPSGS
FMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGG
YLTEAVRRKPYSVILLDEVEKAHPDVFNVLLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
GSVQIQELVGDREAQRAAVMDAVSTHFRPEFINRIDEVVIFEPLARDQIAGITEIQLGRL
RSRLAERELKLTLSSEALDKLIAVGYDPVYGARPLKRAIQRWIENPLAQLILAGSFLPGT
TVTATVANDEIVFN