Protein Info for AO353_14225 in Pseudomonas fluorescens FW300-N2E3

Annotation: laccase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02578: Cu-oxidase_4
  • amino acids 20 to 238 (219 residues), 253.7 bits, E=8.3e-80
• TIGR00726: YfiH family protein
  • amino acids 25 to 239 (215 residues), 202.8 bits, E=2.3e-64

Best Hits

Swiss-Prot: 75% identical to POLOX_PSEAE: Polyphenol oxidase (PA4543) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05810, conserved hypothetical protein (inferred from 86% identity to pfo:Pfl01_4835)

MetaCyc: 57% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]

Predicted SEED Role

"COG1496: Uncharacterized conserved protein"

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• L-methionine salvage cycle III (10/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• purine ribonucleosides degradation (5/6 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• superpathway of purine deoxyribonucleosides degradation (5/7 steps found)
• 5'-deoxyadenosine degradation I (2/3 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• nucleoside and nucleotide degradation (halobacteria) (2/6 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• sesamin biosynthesis (1/8 steps found)
• nucleoside and nucleotide degradation (archaea) (2/10 steps found)
• justicidin B biosynthesis (1/10 steps found)
• matairesinol biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (4/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1, 2.4.2.28, 3.5.4.4

Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4

Sequence Analysis Tools

See A0A0N9VUU3 at UniProt or InterPro

Protein Sequence (241 amino acids)

>AO353_14225 laccase (Pseudomonas fluorescens FW300-N2E3)
MNDWLIPDWPAPAGVKACVTTRAGGVSLAPFDSLNLGDHVDDNPEAVAENRRRLTDHFSI
QPAWLQQVHGVTVAYADPTAVATADASWTATPGIACTAMTADCLPALFCDRAGTRVAAAH
AGWRGLAAGVLEATVESLAVDPAEVLVWLGPAIGPKAFEVGPEVREAFVRQLAETDQAFV
PSHNAGKLMADIYTLARLRLAACGVTAVYGGGFCTVTDPRFFSYRRSPRTGRFASLVWLE
R