Protein Info for AO353_14055 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATP-dependent protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 814 PF20437: LonC_helical" amino acids 92 to 123 (32 residues), 49.3 bits, see alignment (E = 8.1e-17) PF13654: AAA_32" amino acids 317 to 448 (132 residues), 175.2 bits, see alignment E=1.3e-55 PF20436: LonB_AAA-LID" amino acids 476 to 540 (65 residues), 71.2 bits, see alignment E=1.2e-23 PF05362: Lon_C" amino acids 650 to 752 (103 residues), 27.4 bits, see alignment E=4.7e-10

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4872)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type II" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPV4 at UniProt or InterPro

Protein Sequence (814 amino acids)

>AO353_14055 ATP-dependent protease (Pseudomonas fluorescens FW300-N2E3)
MPDPVAASLRLAPEALTRPFSAEQFSFSTTNELEPFRGVLGQERAVEALQFGVAMPRPGY
NVFVMGEPGTGRFSFVKRYLKAEGKRLQTPADWVYVNNFDEPREPRALELPSGTAGAFIT
DINGLIDNLLATFPAVFEHPSYQQKKSAIDRAFNQRYDRALDVIERLALEKEVALYRDST
NIAFTPMSEGKALDEAEFAQLPEAVRERFHDDISGLEERLNEELASLPQWKRESSNQLRQ
LNEETITLALQPLLSPLSEKYAENAAVCGYLQAMQVYLLKTVVEQLVDDSKTDAVARKLL
EEQYGPSLVVGHPHSGGAPVVFEPHPTYDNLFGRIEYSTDQGALYTTYRQLRPGALHRAN
GGFLILEAEKMLSEPFVWDALKRALQSRKLKMESPLGELGRLATVTLTPQHIPLQVKVVI
IGARQLYYTLQDLDPDFQEMFRVLVDFDEDIPMVDESLEQFAQLLKTRTSEEGMAPLTAD
AVARLATYSARLAEHQGRLSARIGDLFQLVSEADFIRDLAGDEMTDAGHIERALKAKATR
TGRVSARILDDMLAGIILIDTAGAAVGKCNGLTVLEVGDSAFGIPARISATVYPGGSGIV
DIEREVNLGQPIHSKGVMILTGYLGSRYAQEFPLAISASIALEQSYGYVDGDSASLGEAC
TLISALSKTPLKQCFAITGSINQFGEVQAVGGVNEKIEGFFRLCEARGLTGEQGAIIPQA
NVATLMLDERVLAAVRAGQFHVYAVRQADEALSLLVGEAAGEPDEEGQFPEGSVNARVVE
RLRVIAEMISEDDLKEAEKEHVQAQEILAAAKPA