Protein Info for AO353_14025 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00229: PAS domain S-box protein
  • amino acids 450 to 581 (132 residues), 49.1 bits, E=6e-17
  • amino acids 582 to 721 (140 residues), 38.5 bits, E=1.1e-13
  • amino acids 726 to 842 (117 residues), 65.6 bits, E=4.6e-22
• PF13426: PAS_9
  • amino acids 473 to 573 (101 residues), 23.7 bits, E=1.7e-08
  • amino acids 598 to 713 (116 residues), 26.7 bits, E=1.9e-09
  • amino acids 733 to 835 (103 residues), 61 bits, E=4e-20
• PF08447: PAS_3
  • amino acids 477 to 568 (92 residues), 41.1 bits, 6e-14
• PF00989: PAS
  • amino acids 726 to 833 (108 residues), 39.3 bits, E=2.1e-13
• PF08448: PAS_4
  • amino acids 728 to 837 (110 residues), 35.7 bits, E=3.1e-12
• TIGR00254: diguanylate cyclase (GGDEF) domain
  • amino acids 844 to 1010 (167 residues), 139 bits, E=1.2e-44
• PF00990: GGDEF
  • amino acids 847 to 1008 (162 residues), 169.6 bits, E=1.7e-53
• PF00563: EAL
  • amino acids 1028 to 1263 (236 residues), 262.8 bits, E=9.1e-82

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_4876)

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine betaine degradation III (7/7 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• glycine betaine degradation I (6/8 steps found)
• folate transformations I (9/13 steps found)
• photorespiration II (6/10 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.1

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See A0A0N9VUC9 at UniProt or InterPro

Protein Sequence (1282 amino acids)

>AO353_14025 diguanylate phosphodiesterase (Pseudomonas fluorescens FW300-N2E3)
LSNVTPPSSVSALSSATGSPLRGTLKGALATLVLLLIGLLLWQLFDQLSETQNSQRQYTI
DYTADLAAQVSLNMTLNAQIALNLLPIVEKPQTVDQQQALLRKLQQSLPDLLSLALLSPS
GKILSDSASDSQDAGYLSELVRRSHAQVHYFSNANDGSVVNLLLHQASGSTRDYWVLRLR
PTFFSSLTKQSDTGIRPLWVVENRINQQIVSRDDGLPSNKPDTVNANDLANSVLVVPLSN
SDWQLRGLFDRQRVFEQLLPVFIGKCLLGLAFSLLPLIALLNMRRRQRQVHDGRRRYRDI
FDGTGVALCVLDLSGLKACLDKAQLHTSEQLQRWLENPEQRRQLLQELRITEVNQVALHL
LNVQSSEQAWKLLIEGGPLDGNAIGNQLLDAVLNQQKQLELEIKLRDTQGRDQHLWLVLH
LPQAQHDAKAVILSISDITSRKIVELSLLEREGFWSDVVRTVPDHLYVQDVISQRMIFSN
HHLGQTLGYSKTELHQMGEYFWEILLHPEDADHYHRLRQEQRQAGYTQLMQCQLRFRHRD
GKWRRFEIREQALARDKHDQVTRIIGVAKDITDQIEASESLRDSEQRYRMLAESISDVIF
STDSKLSLNYVSPSVNAVLGYDVDWIFQNGWQSTIANPQQLTGIYSLMDRVSKALDKPEQ
LAQLRSQVQTQLFLFDCLRADGRKIPIELRLVLVWDEHGAFEGVLGVGRDISQQRRAEKD
LRMAATVFEHSTSAILITDPAGYIVQANEAFSRVSGYEVAQVLDQLPNMLTVDDQQEAHL
RYVLKQLHQHSTWEGEVWLKRRNGEHYPAWVGITAVLDDEGDLASYVCFFSDISERKASE
QRIHRLAYYDALTHLPNRTLFQDRLHTALQSAERQKSWVVLMFLDLDRFKPINDSLGHAA
GDRMLKEMATRLLACVDDDDTVARMGGDEFTLLLQPRASREIALNRAIHVAEQILASLVK
PFVLEGREFFVTASIGIALSPQDGNELSQLMKNADTAMYHAKERGKNNFQFYQADMNASA
LERLELESDLRHALEQNEFVLYYQPQFSGDGKRLTGAEALLRWRHPRRGLVPPGDFIPVL
EELGLVVDVGDWVISEACRQLKTWHQAKVRVPKVSVNISARQFSDGQLGTRIATILKDTG
LPPACLELELTESILMREVSEAMQILAGLKNLGLSIAVDDFGTGYSSLNYLKQFPIDVLK
IDRTFVDGLPSGEQDAQIARAIIAMAHSLNLAVIAEGVETHEQLDFLREHGCDEVQGYLF
GRPMPAARFEAQFSNDALFMFD