Protein Info for AO353_13820 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 PF00437: T2SSE" amino acids 94 to 365 (272 residues), 246.7 bits, see alignment E=1.2e-77

Best Hits

KEGG orthology group: K02283, pilus assembly protein CpaF (inferred from 93% identity to pfl:PFL_0695)

Predicted SEED Role

"Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPR7 at UniProt or InterPro

Protein Sequence (424 amino acids)

>AO353_13820 ATPase (Pseudomonas fluorescens FW300-N2E3)
MSGEKLFGAQTQSPIGNTDHEGLKLVLHRYVIDAIEESGKNLLEGSRPLLAQFVTDKVAE
YIARLHLAISRYEMERLAEEIVDELTGFGPLEVLLRDPAVTEILVNGPHRVFIERDGVLH
QSDLRFIDAHHVERVMQRILAPLGRRLDESSPMVDARMPDGSRVNAIIPPIALDGPCLSI
RKFRKDMLKSSDLMAMQTIDQAIFDFIEKAVGKRCNILISGGTGTGKTTLLNILSQLIDP
HERLVTIEDVAELQLGHPHVVRLETRPPNAEGHGEVKASDLIRNALRMRPDRIILGEIRG
VEVLDVLTAMNTGHDGSMSTVHANNAQDALLRLETLVGLTGRQIAERTLRQMICAALDVV
IQLTRLPDGRRCVSEVVEVVGIREDIYVTNTLFRFDRRTGFGFLREAVNPAGDKLRHELA
LPLN