Protein Info for AO353_13750 in Pseudomonas fluorescens FW300-N2E3

Annotation: SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13489: Methyltransf_23
  • amino acids 30 to 155 (126 residues), 53.6 bits, E=6.9e-18
• PF05175: MTS
  • amino acids 34 to 113 (80 residues), 26.2 bits, E=1.7e-09
• PF13847: Methyltransf_31
  • amino acids 41 to 151 (111 residues), 48.2 bits, E=3e-16
• PF13649: Methyltransf_25
  • amino acids 45 to 138 (94 residues), 52.8 bits, E=1.6e-17
• PF08241: Methyltransf_11
  • amino acids 46 to 141 (96 residues), 54.5 bits, E=4.5e-18
• PF08242: Methyltransf_12
  • amino acids 46 to 140 (95 residues), 53 bits, E=1.4e-17

Best Hits

KEGG orthology group: K06219, S-adenosylmethionine-dependent methyltransferase (inferred from 93% identity to pfl:PFL_0683)

Predicted SEED Role

"3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64)" in subsystem Ubiquinone Biosynthesis (EC 2.1.1.64)

MetaCyc Pathways

• superpathway of ubiquinol-8 biosynthesis (early decarboxylation) (10/12 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• ubiquinol-8 biosynthesis (early decarboxylation) (6/8 steps found)
• ubiquinol-8 biosynthesis (late decarboxylation) (4/9 steps found)
• ubiquinol-6 biosynthesis (late decarboxylation) (3/8 steps found)
• ubiquinol-9 biosynthesis (early decarboxylation) (3/8 steps found)
• ubiquinol-9 biosynthesis (late decarboxylation) (3/8 steps found)
• ubiquinol-6 biosynthesis from 4-aminobenzoate (yeast) (3/9 steps found)
• ubiquinol-10 biosynthesis (early decarboxylation) (2/8 steps found)
• ubiquinol-7 biosynthesis (early decarboxylation) (2/8 steps found)
• ubiquinol-7 biosynthesis (late decarboxylation) (2/8 steps found)
• superpathway of ubiquinol-6 biosynthesis (late decarboxylation) (4/11 steps found)
• ubiquinol-10 biosynthesis (late decarboxylation) (2/9 steps found)

KEGG Metabolic Maps

• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.64

Use Curated BLAST to search for 2.1.1.64

Sequence Analysis Tools

See A0A0N9WK73 at UniProt or InterPro

Protein Sequence (249 amino acids)

>AO353_13750 SAM-dependent methyltransferase (Pseudomonas fluorescens FW300-N2E3)
MSDRHFDQLATRFAEKIYGGAKGAIRLAVLQADLAETLPDRPLRVLDIGAGLGHMSLWLA
QRGHQVTLAEPAEPMLEGARQRFAEAGQAATFIQAPWQELLGQLTEPYDLVLCHAVLEWL
AEPHAILPVLHQLTTKDGWLSLAFYNRDALIYRNLLKGHFRKMRKNDMAGEKQSLTPQMP
LDPRELATQLDGLWQVETQSGVRVFHDYMPVEFQARAELIDLLEMELAHRRHPSFAGLGR
YLHWICRPV