Protein Info for AO353_13735 in Pseudomonas fluorescens FW300-N2E3

Annotation: MATE family efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 17 to 37 (21 residues), see Phobius details amino acids 49 to 71 (23 residues), see Phobius details amino acids 95 to 121 (27 residues), see Phobius details amino acids 141 to 159 (19 residues), see Phobius details amino acids 167 to 187 (21 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details amino acids 247 to 266 (20 residues), see Phobius details amino acids 277 to 296 (20 residues), see Phobius details amino acids 317 to 343 (27 residues), see Phobius details amino acids 359 to 380 (22 residues), see Phobius details amino acids 392 to 410 (19 residues), see Phobius details amino acids 416 to 436 (21 residues), see Phobius details PF01554: MatE" amino acids 23 to 183 (161 residues), 81.2 bits, see alignment E=3.7e-27 amino acids 248 to 405 (158 residues), 51.5 bits, see alignment E=5e-18 TIGR00797: MATE efflux family protein" amino acids 23 to 416 (394 residues), 244.6 bits, see alignment E=8.4e-77

Best Hits

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 90% identity to pfo:Pfl01_0630)

Predicted SEED Role

"DNA-damage-inducible protein F"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WI90 at UniProt or InterPro

Protein Sequence (446 amino acids)

>AO353_13735 MATE family efflux transporter (Pseudomonas fluorescens FW300-N2E3)
MSTLINDWRDRATHRRVWALAAPMILSNISVPLVALVDSTVVGHLPHAHQLGAVAVGASL
YTFLAWAMGFLRMGSTGFAAQAAGRGDGAALRQILLQGLLLAMGLALLLGMLGVPLSGVA
LHFMQPSAELDQLTREFFHTRLFGLPAALASYALVGWFLGTQNARAPLAILLTTNLVNIA
LNLWFVLGLQWGVVGAARASVIAEWTGALLGLAMTRNALRAYPGHIAWAALALWQSWRPL
LAVNRDIFIRSLALQSVFFMVTVQGARLGDATVAANALLLNGLLLTAHALDGLAHAVEAL
CGHAIGARDRQALRRSLVVACGWSLIASLGFALLFFCAGHLFIEMQTNIQSVRDTAFVYL
PYLAALPLIAVWSYLLDGLFIGATRAREMRNGMLLTVLLLLPFAWTLQGFGNHGLWLTFL
LFMVLRSLMLGAFAWNLRKNDGWFTV