Protein Info for AO353_13700 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 PF00072: Response_reg" amino acids 251 to 362 (112 residues), 78.7 bits, see alignment E=3.7e-26 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 373 to 536 (164 residues), 153.3 bits, see alignment E=2.5e-49 PF00990: GGDEF" amino acids 378 to 532 (155 residues), 134.6 bits, see alignment E=2.8e-43

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_0623)

Predicted SEED Role

"Probable two-component response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPQ0 at UniProt or InterPro

Protein Sequence (556 amino acids)

>AO353_13700 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3)
MTEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRCEWSGADLAELSEANLRLLRFAER
FEQPEHTHLAHSISQSLAAVDANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLEQTFLPP
LRKPIYVMLQDHDRAERLAKQLEFFGLSAQSLDSVAAFRSSMVERLPAAIVMDVDFSGPG
MGLQLAAEAQEGLEQKLPLLFFSLLETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVL
TCVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDM
YMPACTGTELAKVIRHNDRYVSVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLIT
TVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFRARRENKPLSFAMLDIDHFKR
VNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIESAYKVLDEIRQ
RFAEIHYPAQPQDLWCTFSAGVVDLHEECDSLMMASQADEALYRAKDAGRNRVQAARSSN
QSATFSPESTDSVITL