Protein Info for AO353_13620 in Pseudomonas fluorescens FW300-N2E3

Annotation: precorrin-3B C17-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF11760: CbiG_N" amino acids 51 to 129 (79 residues), 80.3 bits, see alignment E=8.3e-27 PF00590: TP_methylase" amino acids 313 to 524 (212 residues), 112.2 bits, see alignment E=3.4e-36 TIGR01466: precorrin-3B C17-methyltransferase" amino acids 314 to 560 (247 residues), 284.8 bits, see alignment E=2.9e-89

Best Hits

KEGG orthology group: K13541, cobalamin biosynthesis protein CbiG / precorrin-3B C17-methyltransferase [EC: 2.1.1.131] (inferred from 83% identity to pfo:Pfl01_0607)

Predicted SEED Role

"Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.131

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WVF7 at UniProt or InterPro

Protein Sequence (566 amino acids)

>AO353_13620 precorrin-3B C17-methyltransferase (Pseudomonas fluorescens FW300-N2E3)
MTRSVPAIVILGNGSLATARKIQQLYPGALIHGLAERVDGADRVYHEFGATLRQLYQHDT
PIIALCAAGIVIRTLAPLLLEKGAEPPVLAVAEDGSAVVPLLGGLGGVNVLAREIADGLG
VAAAITTSGELRFGTCLLNPPSGYALGDLELGKRFVSDLLAGESVRIEGAAPWLDQAQLP
QDPQAHLAIHIGSAERAPAANELRIYPQNVLVAVSADVADLPNAIRDALHQAEIAVQSLA
CLLASDQQMASLALREAALELGVPLRFATAGSVAELAENAVGQSMFIDTSNGIAIAVADR
PLDPSLIGRPRGRLAVIGLGPGAAELMVPAVKAELARANDVLGYETYVRMAGPFRADQVL
HCTDNREEMQRARHAFELAVQGRSVVVVSSGDPGVFAMAAAVLEALHESSDASWHSVDLE
ILPGVSASLATAAQAGAPLGHDFCVMSLSDNLKPWAIIEKRLDLAAEADLALAFYNPISR
SRPWQLGRALEIVARHRTPETPVVLGRDIGRPGQTLRVTTLGQLTPDQVDMRTMVLIGSS
TTCVFPRAEGGEWVYTPRWYSTKPVS